Product Pathways - Translational Control
Ribosomal Protein L7a (E109) Antibody #2415
PhosphoSitePlus® protein, site, and accession data: RPL7A
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R Mk | Endogenous | 32 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Ribosomal protein L7a Antibody detects endogenous levels of total ribosomal protein L7a. Immunofluorescent analysis using this antibody shows exclusive localization of ribosomal protein L7a to nucleoli during ribosomal biosynthesis.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu109 of human ribosomal protein L7a. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines using Ribosomal Protein L7a (E109) Antibody.
IF-IC
Confocal immunofluorescent analysis of COS cells using Ribosomal Protein L7a (E109) Antibody (green). Actin filaments have been labeled with Alexa Fluor® phalloidin 555 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Ribosomes are stained by the antibody at their biosynthesis site in nucleoli.
Background
Ribosomal protein L7a is a highly conserved ribosome protein localized to 60S ribosomal subunit (1). The protein has distinct domains that target the newly synthesized polypeptide to nucleus and the nucleoli, the site of ribosome biosynthesis (2). Ribosomal protein L7a can also interact with RNA in vitro through two distinct RNA-binding domains in the protein (3). Taken together, nucleolar localization and the ability to bind RNA suggests that ribosomal protein L7a may act as an important component for ribosome biosynthesis and function.
- De Falco, S. et al. (1993) Gene 126, 227-235.
- Russo, G. et al. (1997) J. Biol. Chem. 272, 5229-5235.
- Russo, G. et al. (2005) Biochem J. 385, 289-299.
Application References
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Companion Products
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For Research Use Only. Not For Use In Diagnostic Procedures.
