Product Pathways - Development
Notch2 (8A1) Rabbit mAb #2420
Have you tried your application using our XP® monoclonal antibodies? Try products: 4530, 5732
PhosphoSitePlus® protein, site, and accession data: Notch 2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H | Endogenous | 230, 300 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Notch2 (8A1) Rabbit mAb detects endogenous levels of total Notch2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein consisting of the heterodimerization domain of human Notch2.
Background
Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4).
Notch2 is a member of Notch family and mutation in Notch2 is associated with Alagille syndrome (5).
- Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
- Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
- Schroeter, E.H. et al. (1998) Nature 393, 382-6.
- Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
- McDaniell, R. et al. (2006) Am J Hum Genet 79, 169-73.
Application References
- Whyte, J.L. et al. (2011) Stem Cell Res 6, 238-50. Applications: Western Blotting
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.