Cell Signaling Technology

Product Pathways - Development

Notch2 (8A1) Rabbit mAb #2420

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 230, 300 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Notch2 (8A1) Rabbit mAb detects endogenous levels of total Notch2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein consisting of the heterodimerization domain of human Notch2.

Western Blotting

Western Blotting

Western blot analysis of extracts from SNB19 and 293 cells, untreated or stably expressing human Notch2, using Notch2 (8A1) Rabbit mAb. The full-length (FL) and the extracellular domain (EC) of Notch2 are indicated.

IP

IP

Immunoprecipitation of endogenous Notch2 from 8MGBA cell lysates using Notch2 (8A1) Rabbit mAb. Lane 1 contains lysate input and lane 2 was immunoprecipitated with Notch2 (8A1) Rabbit mAb. Western blot detection was performed using the same antibody.

Background

Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4).

Notch2 is a member of Notch family and mutation in Notch2 is associated with Alagille syndrome (5).

  1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
  2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
  3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
  4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
  5. McDaniell, R. et al. (2006) Am J Hum Genet 79, 169-73.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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