Product Pathways - Wnt / Hedgehog / Notch
Notch2 (8A1) Rabbit mAb #2420
Have you tried your application using our XP® monoclonal antibodies? Try products: 4530, 5732
PhosphoSitePlus® protein, site, and accession data: Notch 2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H | Endogenous | 230, 300 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Notch2 (8A1) Rabbit mAb detects endogenous levels of total Notch2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein consisting of the heterodimerization domain of human Notch2.
Western Blotting
Western blot analysis of extracts from SNB19 and 293 cells, untreated or stably expressing human Notch2, using Notch2 (8A1) Rabbit mAb. The full-length (FL) and the extracellular domain (EC) of Notch2 are indicated.
IP
Immunoprecipitation of endogenous Notch2 from 8MGBA cell lysates using Notch2 (8A1) Rabbit mAb. Lane 1 contains lysate input and lane 2 was immunoprecipitated with Notch2 (8A1) Rabbit mAb. Western blot detection was performed using the same antibody.
Background
Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.
Notch2 is a member of Notch family and mutation in Notch2 is associated with Alagille syndrome (5).
- Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
- Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
- Schroeter, E.H. et al. (1998) Nature 393, 382-6.
- Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
- McDaniell, R. et al. (2006) Am J Hum Genet 79, 169-73.
Application References
- Whyte, J.L. et al. (2011) Stem Cell Res 6, 238-50. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2421 Cleaved Notch1 (Val1744) Antibody
- 2423 Notch4 (L5C5) Mouse mAb
- 2588 DLL1 Antibody
- 2589 DLL4 Antibody
- 2483 DLL3 (G93) Antibody
- 2155 Jagged1 (1C4) Rabbit mAb
- 2620 Jagged1 (28H8) Rabbit mAb
- 2210 Jagged2 (C23D2) Rabbit mAb
- 2205 Jagged2 (C83A8) Rabbit mAb
- 4608 MAML1 Antibody
- 4618 MAML2 Antibody
- 2733 Numb Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3268 Notch1 (C44H11) Rabbit mAb
- 4147 Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
