Product Pathways - Wnt / Hedgehog / Notch
Notch2 (8A1) Rabbit mAb #2420
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H | Endogenous | 230, 300 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Notch2 (8A1) Rabbit mAb detects endogenous levels of total Notch2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a recombinant protein consisting of the heterodimerization domain of human Notch2.
Western Blotting
Western blot analysis of extracts from SNB19 and 293 cells, untreated or stably expressing human Notch2, using Notch2 (8A1) Rabbit mAb. The full-length (FL) and the extracellular domain (EC) of Notch2 are indicated.
IP
Immunoprecipitation of endogenous Notch2 from 8MGBA cell lysates using Notch2 (8A1) Rabbit mAb. Lane 1 contains lysate input and lane 2 was immunoprecipitated with Notch2 (8A1) Rabbit mAb. Western blot detection was performed using the same antibody.
Background
Notch1 is a transmembrane protein functioning in development and the determination of cell-fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.
Notch2 is a member of Notch family and mutation in Notch2 is associated with Alagille syndrome (5).
- Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
- Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
- Schroeter, E.H. et al. (1998) Nature 393, 382-6.
- Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
- McDaniell, R. et al. (2006) Am J Hum Genet 79, 169-73.
Application References
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
