Product Pathways - Wnt / Hedgehog / Notch
Cleaved Notch1 (Val1744) Antibody #2421
PhosphoSitePlus® protein, site, and accession data: Notch 1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 110 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Cleaved Notch1 (Val1744) Antibody detects endogenous levels of the cytosolic domain of notch1 only when cleaved between Gly1743 and Val1744. The antibody does not recognize full length notch1 or notch1 cleaved at other positions. This antibody may cross react with overexpressed cleaved notch2, 3 and 4.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence at the Val1744 cleavage site of human notch1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293 cells transfected with full-length Notch1, either untreated or treated with 5 mM EDTA for 15 minutes, and MOLT-4 extracts, using Cleaved Notch 1 (Val1744) Antibody.
Background
Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.
- Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
- Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
- Schroeter, E.H. et al. (1998) Nature 393, 382-6.
- Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
Application References
- Phiel, C. J. et al. (2003) GSK-3alpha regulates production of Alzheimer's disease amyloid-beta peptides. Nature 423, 435-439. Applications: Western Blotting
- Tokunaga, A. et al. (2004) Mapping spatio-temporal activation of Notch signaling during neurogenesis and gliogenesis in the developing mouse brain. J. Neurochem. 90 (1), 142-154. Applications: IC-IF Western Blotting
- Ishikura, N. et al. (2005) Notch-1 activation and dendritic atrophy in prion disease. Proc. Natl. Acad. Sci. USA 102 (3), 886-891. Applications: Western Blotting
- Palomero, T. et al. (2006) Leukemia 20, 1279-87. Applications: Western Blotting
- O'Neil, J. et al. (2007) J Exp Med 204, 1813-24. Applications: Western Blotting
- Huppert, S.S. et al. (2005) Dev Cell 8, 677-88. Applications: Western Blotting
- Köchert, K. et al. (2011) Oncogene 30, 1831-40. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
