Product Pathways - Wnt / Hedgehog / Notch
Cleaved Notch1 (Val1744) Antibody #2421
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 110 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Cleaved Notch1 (Val1744) Antibody detects endogenous levels of the cytosolic domain of notch1 only when cleaved between Gly1743 and Val1744. The antibody does not recognize full length notch1 or notch1 cleaved at other positions. This antibody may cross react with overexpressed cleaved notch2, 3 and 4.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to the sequence at the Val1744 cleavage site of human notch1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293 cells transfected with full-length Notch1, either untreated or treated with 5 mM EDTA for 15 minutes, and MOLT-4 extracts, using Cleaved Notch 1 (Val1744) Antibody.
Background
Notch1 is a transmembrane protein functioning in development and the determination of cell-fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.
- Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
- Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
- Schroeter, E.H. et al. (1998) Nature 393, 382-6.
- Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
Application References
- Phiel, C. J. et al. (2003) GSK-3alpha regulates production of Alzheimer's disease amyloid-beta peptides. Nature 423, 435-439. This article references the use of Cleaved Notch1 (Val1744) Antibody in the following applications: Western Blotting
- Tokunaga, A. et al. (2004) Mapping spatio-temporal activation of Notch signaling during neurogenesis and gliogenesis in the developing mouse brain. J. Neurochem. 90 (1), 142-154. This article references the use of Cleaved Notch1 (Val1744) Antibody in the following applications: IC-IF IHC-FL (floating/frozen) Western Blotting
- Ishikura, N. et al. (2005) Notch-1 activation and dendritic atrophy in prion disease. Proc. Natl. Acad. Sci. USA 102 (3), 886-891. This article references the use of Cleaved Notch1 (Val1744) Antibody in the following applications: Western Blotting
- Palomero, T. et al. (2006) Leukemia 20, 1279-87. This article references the use of Cleaved Notch1 (Val1744) Antibody in the following applications: Western Blotting
- O'Neil, J. et al. (2007) J Exp Med 204, 1813-24. This article references the use of Cleaved Notch1 (Val1744) Antibody in the following applications: Western Blotting
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
