Product Pathways - Development
Cleaved Notch1 (Val1744) Antibody #2421
|2421L||300 µl (30 western blots)||---||In Stock||---|
|2421S||100 µl (10 western blots)||---||In Stock||---|
|2421||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||110||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Cleaved Notch1 (Val1744) Antibody detects endogenous levels of the Notch1 intracellular domain (NICD) only when released by cleavage between Gly1753 and Val1754 (equivalent to Gly1743/Val1744 of murine Notch1). This antibody does not recognize full-length Notch1 or Notch1 cleaved at other positions. This antibody may cross-react with overexpressed cleaved Notch2, 3 and 4.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence at the Val1754 cleavage site in human Notch 1 (equivalent to Val1744 in mouse Notch1). Antibodies are purified by protein A and peptide affinity chromatography.
Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4).
The NICD of murine Notch1 is released (activated) by cleavage between Gly1743 and Val1744 (corresponding to Gly1753/Val1754 in human Notch1) (3, 4). Mutations that result in constitutive activation of Notch1 are associated with many different cancers, including a majority of cases of T cell acute lymphoblastic leukemia (T-ALL). Activation may be due to mutations in Notch1 itself, or in components of the ubiquitin ligase complex that negatively regulates the Notch signaling pathway (5-6).
- Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
- Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
- Schroeter, E.H. et al. (1998) Nature 393, 382-6.
- Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
- Weng, A.P. et al. (2004) Science 306, 269-71.
- Thompson, B.J. et al. (2007) J Exp Med 204, 1825-35.
- Phiel, C.J. et al. (2003) Nature 423, 435-9. Applications: Western Blotting.
- Tokunaga, A. et al. (2004) . J. Neurochem. 90 (1), 142-154. Applications: Western Blotting, IC-IF.
- Ishikura, N. et al. (2005) . Proc. Natl. Acad. Sci. USA 102 (3), 886-891. Applications: Western Blotting.
- Palomero, T. et al. (2006) Leukemia 20, 1279-87. Applications: Western Blotting.
- O'Neil, J. et al. (2007) J Exp Med 204, 1813-24. Applications: Western Blotting.
- Huppert, S.S. et al. (2005) Dev Cell 8, 677-88. Applications: Western Blotting.
- Köchert, K. et al. (2011) Oncogene 30, 1831-40. Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3622 Presenilin 1 Antibody
- 2192 Presenilin 2 Antibody
- 3632 Nicastrin Antibody
- 4147 Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb
- 3608 Notch1 (D1E11) XP® Rabbit mAb
- 4380 Notch1 (D6F11) XP® Rabbit mAb
- 3447 Notch1 (5B5) Rat mAb
- 2889 Notch3 Antibody
- 2420 Notch2 (8A1) Rabbit mAb
- 3446 Notch3 (8G5) Rat mAb
- 2423 Notch4 (L5C5) Mouse mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.