Cell Signaling Technology

Product Pathways - Development

Notch4 (L5C5) Mouse mAb #2423

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Transfected Only 80 cleaved. 230 full-length. Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Notch4 (L5C5) Mouse mAb detects levels of the transfected intracellular region of human Notch4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein consisting of the Ankyrin repeat domain of human Notch4.

Western Blotting

Western Blotting

Western blot analysis of the recombinant human ankyrin repeat domains of Notch1, 2, 3 and 4 (HANK1-4, lanes 1-4, respectively), using 5 ng of protein per lane and Notch4 (L5C5) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of total cell lysate of COS cells either untransfected or transiently transfected with a construct expressing the intracellular domain of human Notch4 (ICN4), using Notch4 (L5C5) Mouse mAb.

Background

Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4).

Notch4 is primarily expressed in endothelial cells and is processed similarly to Notch1 (5-7). The mouse Notch4 gene (also known as Int-3) is a frequent target in Mouse Mammary Tumour Virus (MMTV)-induced mammary tumors (6,8). The intracellular domain of Notch4 binds to Smad3 and inhibits TGF-β signaling (9).

  1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
  2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
  3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
  4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
  5. Uyttendaele, H. et al. (1996) Development 122, 2251-2259.
  6. Shirayoshi, Y. et al. (1997) Genes Cells 2, 213-224.
  7. Baron, M. (2003) Semin. Cell Dev. Biol. 14, 113-119.
  8. Gallahan, D. and Callahan, R. (1997) Oncogene 14, 1883-1890.
  9. Sun, Y. et al. (2005) Oncogene 24, 5365-5374.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products