Product Pathways - Wnt / Hedgehog / Notch
Notch4 (L5C5) Mouse mAb #2423
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H | Transfected Only | 80 cleaved. 230 full-length. | Mouse IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Notch4 (L5C5) Mouse mAb detects levels of the transfected intracellular region of human Notch4 protein.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a recombinant protein consisting of the Ankyrin repeat domain of human Notch4.
Western Blotting
Western blot analysis of the recombinant human ankyrin repeat domains of Notch1, 2, 3 and 4 (HANK1-4, lanes 1-4, respectively), using 5 ng of protein per lane and Notch4 (L5C5) Mouse mAb.
Western Blotting
Western blot analysis of total cell lysate of COS cells either untransfected or transiently transfected with a construct expressing the intracellular domain of human Notch4 (ICN4), using Notch4 (L5C5) Mouse mAb.
Background
Notch1 is a transmembrane protein functioning in development and the determination of cell-fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.
Notch4 is primarily expressed in endothelial cells and is processed similarly to Notch1 (5-7). The mouse Notch4 gene (also known as Int-3) is a frequent target in Mouse Mammary Tumour Virus (MMTV)-induced mammary tumors (6,8). The intracellular domain of Notch4 binds to Smad3 and inhibits TGF-β signaling (9).
- Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
- Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
- Schroeter, E.H. et al. (1998) Nature 393, 382-6.
- Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
- Uyttendaele, H. et al. (1996) Development 122, 2251-2259.
- Shirayoshi, Y. et al. (1997) Genes Cells 2, 213-224.
- Baron, M. (2003) Semin. Cell Dev. Biol. 14, 113-119.
- Gallahan, D. and Callahan, R. (1997) Oncogene 14, 1883-1890.
- Sun, Y. et al. (2005) Oncogene 24, 5365-5374.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
