Product Pathways - Translational Control
eIF4A (F52) Antibody #2425
PhosphoSitePlus® protein, site, and accession data: eIF4A1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 48 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2425:
- Western Blotting
Specificity / Sensitivity
eIF4A (F52) Antibody detects endogenous levels of total eIF4A1, eIF4A2 and eIF4A3 proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe52 of human eIF4A. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using eIF4A (F52) Antibody.
Background
A variety of factors contribute to the important biological event of initiation of translation. The eIF4F complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signalling in a translation-independent manner in Drosophila (4,5).
- Rogers, G.W. et al. (2001) J. Biol. Chem. 276, 12598-12608.
- Rogers, G.W. et al. (1999) J. Biol. Chem. 274, 12236-12244.
- Svitkin, Y.V. et al. (2001) RNA 7, 382-394.
- Li, J. and Li, W.X. (2006) Nat. Cell Biol. 8, 1407-1414.
- Affolter, M. and Pyrowolakis, G. (2006) Nat. Cell Biol. 8, 1319-1321.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
