Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

PHB1 Antibody #2426

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 32 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PHB1 Antibody detects endogenous levels of total PHB1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser252 of human PHB1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF-7, Neuro2A, PC12 and COS cells, using PHB1 Antibody.

Background

The prohibitins, called PHB1 and PHB2, are highly conserved proteins that are present in multiple compartments in eukaryotic cells. PHB1 is 30kDa tumor suppressor protein involved in cell cycle control (1). PHB1 has been found in mitochondria, the nucleus and the plasma membrane, as well as extracellularly in circulation (2). In mitochondria prohibitins mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (3,4). In the nucleus prohibitins interact with transcription factors such as Rb and p53 to regulate target gene transcription (2,5). Extracellular prohibitin can bind and activate C3 to enhance complement activation (6).

  1. McClung, J.K. et al. (1995) Exp Gerontol 30, 99-124.
  2. Mishra, S. et al. (2006) J. Cell. Mol. Med. 10, 353-363.
  3. Nijtmans, L.G. et al. (2000) EMBO J. 19, 2444-2451.
  4. Tatsuta, T. et al. (2005) Mol. Biol. Cell 16, 248-259.
  5. Fusaro, G. et al. (2003) J. Biol. Chem. 278, 47853-47861.
  6. Mishra, S. et al. (2007) Mol. Immunol. 44, 1907-1912.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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