Product Pathways - Cytoskeletal Signaling
EML4 Antibody #2428
PhosphoSitePlus® protein, site, and accession data: EML4
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H | Endogenous | 120 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
EML4 Antibody detects endogenous levels of total EML4 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg91 of human EML4. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from MCF7, A431, SHSY5Y and HepG2 cells, using EML4 Antibody.
IF-IC
Confocal immunofluorescent analysis of SH-SY5Y cells using EML4 Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red). Blue psuedocolor = DRAQ5™ (flourescent DNA dye).
Background
EML4 is a 120 kDa microtubule-associated WD-repeat protein of the EMAP (echinoderm microtubule-associated protein) family (1). The expression of EML4 is necessary for correct intracellular microtubule network formation (2). EML4 protein expression is upregulated during mitosis and downregulated during the remaining cell cycle (1). During mitosis, EML4 is heavily phosphorylated and associated with mitotic spindle (2). The amino-terminal segment of EML4 is essential for microtubule association and function.
- Heidebrecht, H.J. et al. (2000) Genomics 68, 348-350.
- Pollmann, M. et al. (2006) Exp. Cell Res. 312, 3241-3251.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
