Product Pathways - Metabolism
Thioredoxin 1 (C63C6) Rabbit mAb #2429
|W IHC-P||H M R||Endogenous||12||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Thioredoxin 1 (C63C6) Rabbit mAb detects endogenous levels of total thioredoxin 1 protein.
Source / Purification
Thioredoxin 1 (C63C6) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Ala9 of human thioredoxin 1.
Western blot analysis of extracts from various cell types using Thioredoxin 1 (C63C6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Thioredoxin 1 (C63C6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Thioredoxin 1 (C63C6) Rabbit mAb in the presence of control peptide (left) or Thioredoxin Blocking Peptide #1057 (right).
Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond which is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (Trx1) and mitochondrial thioredoxin 2 (Trx2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1 (3) Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5).
- Watson, W.H. et al. (2004) Toxicol. Sci. 78, 3-14.
- Burke-Gaffney, A. et al. (2005) Trends Pharmacol. Sci. 26, 398-404.
- Saitoh, M. et al. (1998) EMBO J. 17, 2596-2606.
- Callister, M.E. et al. (2007) Intensive Care Med. 33, 364-367.
- Callister, M.E. et al. (2006) Thorax 61, 521-527.
- Yu, Y. and Richardson, D.R. (2011) J Biol Chem 286, 15413-27. Applications: Western Blotting
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- 2285 Thioredoxin 1 Antibody (Human Specific)
- 2298 Thioredoxin 1 Antibody (Mouse/Rat Preferred)
- 1057 Thioredoxin 1 Blocking Peptide
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- 2770 SOD1 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
For Research Use Only. Not For Use In Diagnostic Procedures.