Cell Signaling Technology

Product Pathways - Metabolism

Thioredoxin 1 (C63C6) Rabbit mAb #2429

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P H M R Endogenous 12 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Thioredoxin 1 (C63C6) Rabbit mAb detects endogenous levels of total thioredoxin 1 protein.

Source / Purification

Thioredoxin 1 (C63C6) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Ala9 of human thioredoxin 1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Thioredoxin 1 (C63C6) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Thioredoxin 1 (C63C6) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Thioredoxin 1 (C63C6) Rabbit mAb in the presence of control peptide (left) or Thioredoxin Blocking Peptide #1057 (right).


Background

Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond which is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (Trx1) and mitochondrial thioredoxin 2 (Trx2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1 (3) Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5).

  1. Watson, W.H. et al. (2004) Toxicol. Sci. 78, 3-14.
  2. Burke-Gaffney, A. et al. (2005) Trends Pharmacol. Sci. 26, 398-404.
  3. Saitoh, M. et al. (1998) EMBO J. 17, 2596-2606.
  4. Callister, M.E. et al. (2007) Intensive Care Med. 33, 364-367.
  5. Callister, M.E. et al. (2006) Thorax 61, 521-527.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products