Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-Shc (Tyr317) Antibody #2431
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M | Endogenous | 50, 55, 70 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Shc (Tyr317) Antibody detects Shc only when phosphorylated at tyrosine 317.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr317 of human Shc. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from A431 and HepG2 cells, untreated or EGF-treated (100 ng/ml) after 24 hours of serum-starvation, using Phospho-Shc (Tyr317) Antibody (upper) or Shc antibody (lower).
Background
Shc possesses SH2 and PTB domains and serves as a scaffold protein in signaling for a variety of receptor tyrosine kinases. Shc exists in p46, p52 and p66 isoforms, which are produced by using alternative translation initiation sites or a differentially spliced message (1-3). In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317 (4-6). GRB2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway (4). Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development (7).
- Pelicci, G. et al. (1992) Cell 70, 93-104.
- Migliaccio, E. et al. (1997) EMBO J. 16, 706-716.
- Okada, S. et al. (1997) J. Biol. Chem. 272, 28042-28049.
- Salcini, A. E. et al. (1994) Oncogene 9, 2827-2836.
- Gotoh, N. et al. (1996) EMBO J. 15, 6197-6204.
- Van der Geer, P. et al. (1996) Curr. Biol. 6, 1435-1444.
- Zhang, L. et al. (2002) Nat. Immunol. 3, 749-755.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9141 Phospho-TrkA (Tyr490) Antibody
- 2242 HER2/ErbB2 Antibody
- 2241 Phospho-HER2/ErbB2 (Tyr877) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2434 Phospho-Shc (Tyr239/240) Antibody
- 2432 Shc Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
