Cell Signaling Technology

Product Pathways - Translational Control

Calnexin Antibody #2433

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P IF-IC H Endogenous 90 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

Calnexin Antibody detects endogenous levels of total calnexin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence around Ala51 of human calnexin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Calnexin Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Calnexin Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Calnexin Antibody (green). Actin filaments have been labeled with Alexa Fluor(R) 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorecent DNA dye).


Background

Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. Calnexin was first identified as being involved in the assembly of murine class I histocompatibility molecules (1,2). Calnexin is a calcium-binding protein embedded in the ER membrane that retains the newly synthesized glycoproteins inside the ER to ensure proper folding and quality control (3-5). The specificity of calnexin for a subset of glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein (5).

  1. Degen, E. and Williams, D.B. (1991) J. Cell Biol. 112, 1099-1115.
  2. Ahluwalia, N. et al. (1992) J. Biol. Chem. 267, 10914-10918.
  3. Rajagopalan, S. et al. (1994) Science 263, 387-390.
  4. Bergeron, J.J. et al. (1994) Trends Biochem. Sci. 19, 124-128.
  5. Williams, D.B. (2006) J. Cell Sci. 119, 615-623.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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