Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Shc (Tyr239/240) Antibody #2434

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 50, 55, 70 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Shc (Tyr239/240) Antibody detects Shc only when phosphorylated at tyrosine 239/240. The antibody may cross-react with activated EGF receptor protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr239/240 of human Shc. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HepG2 cells, untreated or EGF-treated (100 ng/ml) 18 hours of serum-starvation, and Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-Shc (Tyr239/240) Antibody (upper) or Shc antibody #2432 (lower).

Background

Shc possesses SH2 and PTB domains and serves as a scaffold protein in signaling for a variety of receptor tyrosine kinases. Shc exists in p46, p52 and p66 isoforms, which are produced by using alternative translation initiation sites or a differentially spliced message (1-3). In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317 (4-6). GRB2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway (4). Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development (7).

  1. Pelicci, G. et al. (1992) Cell 70, 93-104.
  2. Migliaccio, E. et al. (1997) EMBO J. 16, 706-716.
  3. Okada, S. et al. (1997) J. Biol. Chem. 272, 28042-28049.
  4. Salcini, A. E. et al. (1994) Oncogene 9, 2827-2836.
  5. Gotoh, N. et al. (1996) EMBO J. 15, 6197-6204.
  6. Van der Geer, P. et al. (1996) Curr. Biol. 6, 1435-1444.
  7. Zhang, L. et al. (2002) Nat. Immunol. 3, 749-755.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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