Product Pathways - Nuclear Receptor Signaling
PPARγ (C26H12) Rabbit mAb #2435
|2435S||100 µl (10 western blots)||---||In Stock||---|
|2435||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||53, 57||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
Species predicted to react based on 100% sequence homology: Rat.
Specificity / Sensitivity
PPARγ (C26H12) Rabbit mAb detects endogenous levels of total PPARγ protein.
Source / Purification
PPARγ (C26H12) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Asp69 of human PPARγ.
Western blot analysis of extracts from NIH/3T3 and 3T3-L1 cells (differentiated 6 days) using PPARγ (C26H12) Rabbit mAb.
Immunohistochemical analysis of 3T3-L1 cells, undifferentiated (left) or differentiated (right) , using PPARγ (C26H12) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse brown fat using PPARγ (C26H12) Rabbit mAb.
Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1). PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2). Besides its role in mediating adipogenesis and lipid metabolism (2), PPARγ also modulates insulin sensitivity, cell proliferation and inflammation (3). PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84 (4,5).
- Tontonoz, P. et al. (1995) Curr. Opin. Genet. Dev. 5, 571-576.
- Rosen, E.D. et al. (1999) Mol. Cell 4, 611-617.
- Murphy, G.J. and Holder, J.C. (2000) Trends Pharmacol. Sci. 21, 469-474.
- Camp, H.S. and Tafuri, S.R. (1997) J. Biol. Chem. 272, 10811-10816.
- Adams, M. et al. (1997) J. Biol. Chem. 272, 5128-5132.
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For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5® is a registered trademark of Biostatus Limited.
BODIPY® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.