Product Pathways - Metabolism
Pdx1 Antibody #2437
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PhosphoSitePlus® protein, site, and accession data: PDX1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | M R (H) | Endogenous | 42 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2437:
- Western Blotting
Specificity / Sensitivity
Pdx1 Antibody detects endogenous levels of total Pdx1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human Pdx1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from INS-1 cells using Pdx1 Antibody.
Background
The pancreatic duodenal homeobox gene-1 (Pdx1) is a transcription factor that contributes to pancreas development, pancreatic β-cell differentiation, and mature β-cell function (1,2). It plays an essential role in the commitment of endoderm to a pancreatic and later β-cell phenotype (2,3). In the mature pancreas, Pdx1 expression is more restricted to the pancreatic β-cells (3), where it promotes the expression of genes important for β-cell functions such as insulin, glucokinase, and Glut2 (4-6). Mutations of the corresponding Pdx1 gene may be associated with diabetes and cases of pancreatic insufficiency (7).
- Kaneto, H. et al. (2008) Endocr J 55, 235-52.
- Habener, J.F. et al. (2005) Endocrinology 146, 1025-34.
- Guz, Y. et al. (1995) Development 121, 11-8.
- Ohlsson, H. et al. (1993) EMBO J 12, 4251-9.
- Watada, H. et al. (1996) Diabetes 45, 1826-31.
- Waeber, G. et al. (1996) Mol Endocrinol 10, 1327-34.
- Cockburn, B.N. et al. (2004) J Clin Endocrinol Metab 89, 971-8.
Application References
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Companion Products
- 5679 Pdx1 (D59H3) XP® Rabbit mAb
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
