Cell Signaling Technology

Product Pathways - Glucose Metabolism

Pdx1 Antibody #2437

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-F IF-IC R (H) (M) Endogenous 40 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-F=Immunofluorescence (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Pdx1 Antibody detects endogenous levels of total Pdx1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human Pdx1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from INS-1 cells using Pdx1 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of INS-1 cells using Pdx1 Antibody (green). Actin filaments were labeled with DY-554 Phalloidin (red).

IF-F

IF-F

Confocal immunofluorescent analyis of normal rat pancreas using Pdx1 Antibody (green, left) or Insulin (C27C9) Rabbit mAb #3014 (green, right). Keratin filaments were labeled with Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) #4528 (blue). Red = Propidium Iodide/RNase (fluorescent DNA dye).


Background

The pancreatic duodenal homeobox gene-1 (Pdx1) is a transcription factor that contributes to pancreas development, pancreatic β-cell differentiation and mature β-cell function (1,2). It plays an essential role in the commitment of endoderm to a pancreatic and later β-cell phenotype (2,3). In the mature pancreas, Pdx1 expression is more restricted to the pancreatic β-cells (3), where it promotes the expression of genes important for β-cell functions such as insulin, glucokinase and Glut2 (4-6). Mutations of the corresponding Pdx1 gene may be associated with diabetes and cases of pancreatic insufficiency (7).

  1. Kaneto, H. et al. (2008) Endocr J 55, 235-52.
  2. Habener, J.F. et al. (2005) Endocrinology 146, 1025-34.
  3. Guz, Y. et al. (1995) Development 121, 11-8.
  4. Ohlsson, H. et al. (1993) EMBO J 12, 4251-9.
  5. Watada, H. et al. (1996) Diabetes 45, 1826-31.
  6. Waeber, G. et al. (1996) Mol Endocrinol 10, 1327-34.
  7. Cockburn, B.N. et al. (2004) J Clin Endocrinol Metab 89, 971-8.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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