Cell Signaling Technology

Product Pathways - Glucose Metabolism

SCD1 (M38) Antibody #2438

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC M (H) Endogenous 37 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SCD1 (M38) Antibody detects endogenous levels of total SCD1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding M38 of human SCD1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of 3T3-L1 cell lysates, using SCD1 (M38) Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of 3T3-L1 cells, using SCD1 (M38) Antibody (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Stearoyl-CoA desaturase 1 (SCD1) is a key lipogenic enzyme found in the endoplasmic reticulum that catalyzes the conversion of palmitoyl–CoA and stearoyl–CoA to palmitoleoyl–CoA (16:1) and oleoyl–CoA (18:1) (1-3). Palmitoleate and oleate are the major components of triglycerides, membrane phospholipids and cholesterol esters (1). SCD1-knockout mice show improved insulin sensitivity and reduced body fat (1). Disruption of SCD1 in mouse brown adipose tissue strengthens insulin signaling and results in increased translocation of Glut4 to plasma membrane and enhanced uptake of glucose (4). Furthermore, SCD1 is essential for the onset of diet-induced body weight gain (1) and insulin resistance in the liver (5).

  1. Ntambi, J.M. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 11482-114866.
  2. Kato, H. et al. (2006) J. Cell. Sci. 119, 2342-2353.
  3. Ozols, J. (1997) Mol. Biol. Cell 8, 2281-2290.
  4. Rahman, S.M. et al. (2005) Am. J Physiol. Endocrinol. Metab. 288, E381-387.
  5. Gutiérrez-Juárez, R. et al. (2006) J. Clin. Invest. 116, 1686-1695.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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