Product Pathways - Glucose Metabolism
ATGL (30A4) Rabbit mAb #2439
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC | M | Endogenous | 54 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
M=Mouse
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
ATGL (30A4) Rabbit mAb detects endogenous levels of total ATGL protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence around Pro469 of mouse ATGL.
Western Blotting
Western blot analysis of extracts from NIH/3T3 and differentiated NIH/3T3-L1 cells, using ATGL (30A4) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of NIH/3T3-L1 cells undifferentiated (left) or differentiated (right), showing induced staining of adipocytes, using ATGL (30A4) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse brown fat, using ATGL (30A4) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse lung, showing staining specific to adipocytes, using ATGL (30A4) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of 3T3-L1 cells, using ATGL (30A4) Rabbit mAb (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues (1-3). Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific (4). ATGL was independently identified as desnutrin (5) and the TG-hydrolace inducible phospholipase-A2-ζ (6).
- Holm, C. et al. (1988) Science 241, 1503-1506.
- Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-537.
- Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-221.
- Zimmermann, R. et al. (2004) Science 306, 1383-1386.
- Villena, J.A. et al. (2004) J. Biol. Chem. 279, 47066-47075.
- Jenkins, C.M. et al. (2004) J. Biol. Chem. 279, 48968-48975.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
