Product Pathways - Metabolism
ATGL (30A4) Rabbit mAb #2439
|W IP IHC-P IF-IC||M||Endogenous||54||Rabbit IgG|
Reactivity Key: M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
ATGL (30A4) Rabbit mAb detects endogenous levels of total ATGL protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro469 of mouse ATGL.
Western blot analysis of extracts from NIH/3T3 and differentiated NIH/3T3-L1 cells, using ATGL (30A4) Rabbit mAb.
Immunohistochemical analysis of NIH/3T3-L1 cells undifferentiated (left) or differentiated (right), showing induced staining of adipocytes, using ATGL (30A4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse brown fat, using ATGL (30A4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse lung, showing staining specific to adipocytes, using ATGL (30A4) Rabbit mAb.
Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues (1-3). Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific (4). ATGL was independently identified as desnutrin (5) and the TG-hydrolace inducible phospholipase-A2-ζ (6).
- Holm, C. et al. (1988) Science 241, 1503-1506.
- Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-537.
- Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-221.
- Zimmermann, R. et al. (2004) Science 306, 1383-1386.
- Villena, J.A. et al. (2004) J. Biol. Chem. 279, 47066-47075.
- Jenkins, C.M. et al. (2004) J. Biol. Chem. 279, 48968-48975.
- Wang, H. et al. (2011) J Biol Chem 286, 15707-15. Applications: IP Western Blotting
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.