Cell Signaling Technology

Product Pathways - Nuclear Receptor Signaling

PPARγ (81B8) Rabbit mAb #2443

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M (R) Endogenous 53, 57 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PPARγ (81B8) Rabbit mAb detects endogenous levels of total PPARγ protein.

Source / Purification

PPARγ (81B8) Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His494 of human PPARγ.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and 3T3-L1 cells (differentiated 6 days) using PPARγ (81B8) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of 3T3-L1 cells, using PPARγ (81B8) Rabbit mAb (red) showing nuclear localization in differentiated cells. Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1). PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2). Besides its role in mediating adipogenesis and lipid metabolism (2), PPARγ also modulates insulin sensitivity, cell proliferation and inflammation (3). PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84 (4,5).

  1. Tontonoz, P. et al. (1995) Curr. Opin. Genet. Dev. 5, 571-576.
  2. Rosen, E.D. et al. (1999) Mol. Cell 4, 611-617.
  3. Murphy, G.J. and Holder, J.C. (2000) Trends Pharmacol. Sci. 21, 469-474.
  4. Camp, H.S. and Tafuri, S.R. (1997) J. Biol. Chem. 272, 10811-10816.
  5. Adams, M. et al. (1997) J. Biol. Chem. 272, 5128-5132.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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