Product Pathways - Nuclear Receptor Signaling
PPARγ (81B8) Rabbit mAb #2443
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC | H M (R) | Endogenous | 53, 57 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
PPARγ (81B8) Rabbit mAb detects endogenous levels of total PPARγ protein.
Source / Purification
PPARγ (81B8) Rabbit mAb is produced by immunizing animals with a synthetic peptide (KLH-coupled) derived from the sequence around His494 of human PPARγ.
Western Blotting
Western blot analysis of extracts from NIH/3T3 and 3T3-L1 cells (differentiated 6 days) using PPARγ (81B8) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of 3T3-L1 cells, using PPARγ (81B8) Rabbit mAb (red) showing nuclear localization in differentiated cells. Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1). PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2). Besides its role in mediating adipogenesis and lipid metabolism (2), PPARγ also modulates insulin sensitivity, cell proliferation and inflammation (3). PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84 (4,5).
- Tontonoz, P. et al. (1995) Curr. Opin. Genet. Dev. 5, 571-576.
- Rosen, E.D. et al. (1999) Mol. Cell 4, 611-617.
- Murphy, G.J. and Holder, J.C. (2000) Trends Pharmacol. Sci. 21, 469-474.
- Camp, H.S. and Tafuri, S.R. (1997) J. Biol. Chem. 272, 10811-10816.
- Adams, M. et al. (1997) J. Biol. Chem. 272, 5128-5132.
Application References
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
