Cell Signaling Technology

Product Pathways - Translational Control

eIF4H Antibody #2444

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M R Mk Endogenous 25, 27 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

eIF4H Antibody detects endogenous levels of total eIF4H protein (both long and short isoforms).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human eIF4H. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using eIF4H Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of PANC-1 cells using eIF4H Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

A variety of factors contribute to the initiation of translation. Eukaryotic translation initiation factor 4H (eIF4H) was purified to near homogeneity from rabbit reticulocyte lysate and shown to stimulate translation in an assay deficient in eIF4F and eIF4B (1). eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (1,2). eIF4H was further shown to stimulate the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (3). Interaction between eIF4H and the herpes simplex virus shutoff protein (Vhs) appears to be important for Vhs-mediated degradation of mRNA (4). Deletion of a large region of chromosome 7, including the corresponding eIF4H gene, results in Williams-Beuren Syndrome (WBS), an autosomal dominant disorder that can present with cardiovascular problems, mental retardation and distinctive facial features (5).

  1. Richter-Cook, N.J. et al. (1998) J Biol Chem 273, 7579-87.
  2. Rogers, G.W. et al. (1999) J Biol Chem 274, 12236-44.
  3. Rogers, G.W. et al. (2001) J Biol Chem 276, 30914-22.
  4. Sarma, N. et al. (2008) J Virol 82, 6600-9.
  5. Osborne, L.R. et al. (1996) Genomics 36, 328-36.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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