Product Pathways - Translational Control
eIF4H Antibody #2444
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R Mk | Endogenous | 25, 27 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
eIF4H Antibody detects endogenous levels of total eIF4H protein (both long and short isoforms).
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from a sequence of human eIF4H. Antibodies are purified by protein A and peptide affinity chromatography.
Background
A variety of factors contribute to the initiation of translation. Eukaryotic initiation factor 4H (eIF4H) was purified to near homogeneity from rabbit reticulocyte lysate and shown to stimulate translation in an assay deficient in eIF4F and eIF4B (1). eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (1,2). eIF4H was further shown to stimulate the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (3).
- Richter-Cook, N.J. et al. (1998) J Biol Chem 273, 7579-87.
- Rogers, G.W. et al. (1999) J Biol Chem 274, 12236-44.
- Rogers, G.W. et al. (2001) J Biol Chem 276, 30914-22.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2425 eIF4A (F52) Antibody
- 2490 eIF4A1 Antibody
- 3592 eIF4B Antibody
- 9742 eIF4E Antibody
- 2469 eIF4G (C45A4) Rabbit mAb
- 2498 eIF4G Antibody
- 2480 eIF5 Antibody
- 2103 eIF2α (L57A5) Mouse mAb
- 9722 eIF2α Antibody
- 3595 eIF2B-ε Antibody
- 2297 4E-T Antibody
- 9644 4E-BP1 (53H11) Rabbit mAb
- 9452 4E-BP1 Antibody
- 2845 4E-BP2 Antibody
- 4763 Translation Initiation Complex Antibody Sampler Kit
- 9918 Translational Control Sampler Kit
- 9997 Tris Buffered Saline with Tween 20 (TBST - 10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.