Product Pathways - Related Products
Glu-Glu Tag Antibody #2448
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | All | Transfected Only | N/A | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: All=All species expected
Specificity / Sensitivity
Glu-Glu Tag Antibody detects transfected proteins containing the Glu-Glu epitope tag. The antibody recognizes the Glu-Glu tag fused to either the amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (Blue Carrier coupled). Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of total cell lysates from Cos cells (lane 1), Cos cells expressing an amino-terminal Glu-Glu-tagged protein (lane 2), and Cos cells expressing a carboxy-terminal Glu-Glu-tagged protein (lane 3), using Glu-Glu Tag Antibody.
IF-IC
Confocal immunofluorescent analysis of parental COS cells (left), COS cells expressing an amino-terminal Glu-Glu-tagged protein (middle), and COS cells expressing a carboxy-terminal Glu-Glu-tagged protein (right), labeled with Glu-Glu Tag Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
