Product Pathways - Neuroscience

APP/β-Amyloid (NAB228) Mouse mAb #2450

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Applications	Reactivity	MW (kDa)	Source	Isotype
W IHC-P IF-P	H (Mk) (B) (Dg)	100 to 140	Mouse	IgG2a

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-P=Immunofluorescence (Paraffin)
Reactivity Key:  H=Human  Mk=Monkey  B=Bovine  Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

APP/beta-Amyloid (NAB228) Mouse mAb detects endogenous levels of APP/beta-Amyloid protein. Although this antibody recognizes both the phospho and non-phospho forms of the protein, it has been shown to prefer the phosphorylated form in some systems.

Source / Purification

Monoclonal antibody is produced by immunizing mice with beta-amyloid and the epitope maps to the amino terminus of beta-amyloid (Lee et al., 2003).

Background

Amyloid beta (A4) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein existing as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (A-beta), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released A-beta fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion pathway of this protein (2-5). The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle dependent (G2/M-phase) (4). The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).

1. Selkoe, D.J. (1996) J. Biol. Chem. 271, 18295-18298.
2. Caporaso, G.L. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 3055-3059.
3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J. 13, 534-542.
4. Suzuki, T. et al. (1994) EMBO J. 13, 1114-1122.
5. Ando, K. et al. (1999) J. Neurosci. 19, 4421-4427.
6. Iijima, K.I. et al. (2000) J. Neurochem. 75, 1085-1091.

Application References

• Lee, E.B. et al. (2003) Secretion and intracellular generation of truncated Abeta in beta-site amyloid-beta precursor protein-cleaving enzyme expressing human neurons. J. Biol. Chem. 278, 4458-4466. This article references the use of APP/β-Amyloid (NAB228) Mouse mAb in the following applications: Western Blotting
• Lee, E.B. et al. (2005) BACE overexpression alters the subcellular processing of APP and inhibits Abeta deposition in vivo. J.Cell. Biol. 168, 291-302. This article references the use of APP/β-Amyloid (NAB228) Mouse mAb in the following applications: IHC-P (paraffin)
• Lee, E.B. et al. (2006) J. Biol. Chem. 281, 4292-4299. This article references the use of APP/β-Amyloid (NAB228) Mouse mAb in the following applications: IHC-P (paraffin) Western Blotting

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