Product Pathways - Neuroscience
APP/β-Amyloid (NAB228) Mouse mAb #2450
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IHC-P IF-P | H (Mk) (B) (Dg) | 100 to 140 | Mouse | IgG2a |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-P=Immunofluorescence (Paraffin)
Reactivity Key:
H=Human
Mk=Monkey
B=Bovine
Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
APP/beta-Amyloid (NAB228) Mouse mAb detects endogenous levels of APP/beta-Amyloid protein. Although this antibody recognizes both the phospho and non-phospho forms of the protein, it has been shown to prefer the phosphorylated form in some systems.
Source / Purification
Monoclonal antibody is produced by immunizing mice with beta-amyloid and the epitope maps to the amino terminus of beta-amyloid (Lee et al., 2003).
Western Blotting
Western blot analysis of extracts from HeLa and SK-N-MC cells, using APP/beta-Amyloid (NAB228) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded Alzheimer's brain, using APP/beta-Amyloid (NAB228) Mouse mAb.
IF-P
Confocal immunofluorescent analysis of paraffin-embedded human Alzheimer's brain using APP/β-Amyloid (NAB228) Mouse mAb (green) and Phospho-p44/42 MAPK (Thr202/Tyr204) (197G2) Rabbit mAb #4377 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Amyloid beta (A4) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein existing as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (A-beta), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released A-beta fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion pathway of this protein (2-5). The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle dependent (G2/M-phase) (4). The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).
- Selkoe, D.J. (1996) J. Biol. Chem. 271, 18295-18298.
- Caporaso, G.L. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 3055-3059.
- Hung, A.Y. and Selkoe, D.J. (1994) EMBO J. 13, 534-542.
- Suzuki, T. et al. (1994) EMBO J. 13, 1114-1122.
- Ando, K. et al. (1999) J. Neurosci. 19, 4421-4427.
- Iijima, K.I. et al. (2000) J. Neurochem. 75, 1085-1091.
Application References
- Lee, E.B. et al. (2003) Secretion and intracellular generation of truncated Abeta in beta-site amyloid-beta precursor protein-cleaving enzyme expressing human neurons. J. Biol. Chem. 278, 4458-4466. This article references the use of APP/β-Amyloid (NAB228) Mouse mAb in the following applications: Western Blotting
- Lee, E.B. et al. (2005) BACE overexpression alters the subcellular processing of APP and inhibits Abeta deposition in vivo. J.Cell. Biol. 168, 291-302. This article references the use of APP/β-Amyloid (NAB228) Mouse mAb in the following applications: IHC-P (paraffin)
- Lee, E.B. et al. (2006) J. Biol. Chem. 281, 4292-4299. This article references the use of APP/β-Amyloid (NAB228) Mouse mAb in the following applications: IHC-P (paraffin) Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2451 Phospho-APP (Thr668) Antibody
- 2452 APP Antibody
- 2454 β-Amyloid Antibody
- 4019 Tau (Tau46) Mouse mAb
- 3622 Presenilin 1 Antibody
- 2644 α/β-Synuclein (Syn205) Mouse mAb
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody