Product Pathways - Neuroscience
β-Amyloid Antibody #2454
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IHC-P IF-P | H | 5 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-P=Immunofluorescence (Paraffin)
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
beta-Amyloid Antibody detects several isoforms of beta-amyloid peptide (Abeta), such as Abeta-40, Abeta-42 etc, regardless of phosphorylation state.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues at the amino terminus of human beta-amyloid peptides. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of human A-beta-40 and A-beta-42 peptides (1-5 ng), using beta-Amyloid Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded Alzheimer's brain, using beta-Amyloid Antibody.
IF-P
Confocal immunofluorescent analysis of human Alzheimer's brain, using beta-Amyloid Antibody (red) and Tau (Tau46) Mouse mAb #4019 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Amyloid beta (A4) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein existing as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (A-beta), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released A-beta fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion pathway of this protein (2-5). The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle dependent (G2/M-phase) (4). The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).
- Selkoe, D.J. (1996) J. Biol. Chem. 271, 18295-18298.
- Caporaso, G.L. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 3055-3059.
- Hung, A.Y. and Selkoe, D.J. (1994) EMBO J. 13, 534-542.
- Suzuki, T. et al. (1994) EMBO J. 13, 1114-1122.
- Ando, K. et al. (1999) J. Neurosci. 19, 4421-4427.
- Iijima, K.I. et al. (2000) J. Neurochem. 75, 1085-1091.
Application References
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Companion Products
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2451 Phospho-APP (Thr668) Antibody
- 2452 APP Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack