Cell Signaling Technology

Product Pathways - Neuroscience

β-Amyloid Antibody #2454

Applications Reactivity MW (kDa) Source
W IHC-P IF-P H 5 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-P=Immunofluorescence (Paraffin)
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

beta-Amyloid Antibody detects several isoforms of beta-amyloid peptide (Abeta), such as Abeta-40, Abeta-42 etc, regardless of phosphorylation state.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues at the amino terminus of human beta-amyloid peptides. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of human A-beta-40 and A-beta-42 peptides (1-5 ng), using beta-Amyloid Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Alzheimer's brain, using beta-Amyloid Antibody.

IF-P

IF-P

Confocal immunofluorescent analysis of human Alzheimer's brain, using beta-Amyloid Antibody (red) and Tau (Tau46) Mouse mAb #4019 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


Background

Amyloid beta (A4) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein existing as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (A-beta), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released A-beta fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion pathway of this protein (2-5). The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle dependent (G2/M-phase) (4). The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).

  1. Selkoe, D.J. (1996) J. Biol. Chem. 271, 18295-18298.
  2. Caporaso, G.L. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 3055-3059.
  3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J. 13, 534-542.
  4. Suzuki, T. et al. (1994) EMBO J. 13, 1114-1122.
  5. Ando, K. et al. (1999) J. Neurosci. 19, 4421-4427.
  6. Iijima, K.I. et al. (2000) J. Neurochem. 75, 1085-1091.

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