Product Pathways - Cytoskeletal Signaling
Phospho-Rac1/cdc42 (Ser71) Antibody #2461
|2461S||100 µl (10 western blots)||---||In Stock||---|
|2461P||40 µl (4 western blots)||---||In Stock||---|
|2461||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Mouse, Rat.
Specificity / Sensitivity
Phospho-Rac1/cdc42 (Ser71) Antibody detects endogenous levels of Rac1/cdc42 only when phosphorylated at serine 71. The antibody may also recognize phospho-RhoA (Ser73).
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser71 of human Rac1/cdc42. Antibodies are purified by protein A and peptide affinity chromatography.
Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development (1). GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3).
A putative Akt phosphorylation site at Ser71 of Rac1/cdc42 has been identified and confirmed by in vitro kinase assay (4). Phosphorylation at this site may inhibit GTP binding of Rac1, attenuating the signal transduction pathway downstream of Rac1 (4).
- Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
- Bernards, A. and Settleman, J. (2004) Trends Cell Biol 14, 377-85.
- Rossman, K.L. et al. (2005) Nat Rev Mol Cell Biol 6, 167-80.
- Kwon, T. et al. (2000) J. Biol. Chem. 275, 423-428.
- John, G. R. et al. (2004) . J. Neurosci. 24 (11), 2837-2845. Applications: Western Blotting.
- Faraone, D. et al. (2006) Blood 107, 1896-902. Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2601 Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody
- 2602 PAK1 Antibody
- 2604 PAK1/2/3 Antibody
- 2605 Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
- 2606 Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody
- 2607 Phospho-PAK2 (Ser20) Antibody
- 3242 PAK4 Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 2466 Cdc42 (11A11) Rabbit mAb
- 2462 Cdc42 Antibody
- 2465 Rac1/2/3 Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.