Cell Signaling Technology

Product Pathways - Development

PTCH2 (L849) Antibody #2464

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Transfected Only 130 kDa Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PTCH2 (L849) Antibody detects transfected levels of PTCH2 protein. It does not recognize transfected levels of human PTCH1. This antibody also cross-reacts with an unidentified protein at 55 kDa. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region (predicted to be extracellular) surrounding residue Leu849 of human PTCH2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from COS cells and COS cells transiently transfected with a construct expressing human PTCH2 using PTCH2 (L849) Antibody.

Background

Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the receiving receptors for members of the Hedgehog family of proteins (1-4). In the absence of Hedgehog proteins, PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) so that the Hedgehog signaling pathway is in the off state (5,6). Deactivating mutations that impair the ability of PTCH1 to suppress Smo are frequently found in patients with nevoid basal cell carcinoma syndrome (7,8). PTCH proteins have a sterol-sensing domain (SSD) also found in several proteins that function in cholesterol homeostasis, such as HMGCR (3-hydroxy-3-methylglutaryl coenzyme A-reductase) and SCAP (sterol regulatory element-binding protein-cleavage activating protein). However, the role of the SSD in Patched proteins is not clear (9,10).

  1. Stone, D.M. et al. (1996) Nature 384, 129-134.
  2. Chen, Y. and Struhl, G. (1996) Cell 87, 553-563.
  3. Motoyama, J. et al. (1998) Nat. Genet. 18, 104-106.
  4. Smyth, I. et al. (1999) Hum. Mol. Genet. 8, 291-297.
  5. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
  6. McMahon, A.P. et al. (2003) Curr. Top. Dev. Biol. 53, 1-114.
  7. Hahn, H. et al. (1996) Cell 85, 841-851.
  8. Johnson, R.L. et al. (1996) Science 272, 1668-1671.
  9. Kuwabara, P.E. and Labouesse, M. (2002) Trends Genet. 18, 193-201.
  10. Chang, T.Y. et al. (2006) Annu. Rev. Cell Dev. Biol. 22, 129-157.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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