Cell Signaling Technology

Product Pathways - Development

PTCH1 (C53A3) Rabbit mAb #2468

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Transfected Only 180-210 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PTCH1 (C53A3) Rabbit mAb detects transfected levels of PTCH1. This antibody can also detect endogenous levels of PTCH1 through immunoprecipitation and followed by Western blot analysis.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1307 of human PTCH1.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from COS cells, untransfected or transiently transfected with a human PTCH1 expression construct, using PTCH1 (C53A3) Rabbit mAb.

IP

IP

Immunoprecipitation of endogenous PTCH1 from 293 cell lysates using PTCH1 (C53A3) Rabbit mAb. Western blot detection was performed using the same antibody.

Background

Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the receiving receptors for members of the Hedgehog family of proteins (1-4). In the absence of Hedgehog proteins, PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) so that the Hedgehog signaling pathway is in the off state (5,6). Deactivating mutations that impair the ability of PTCH1 to suppress Smo are frequently found in patients with nevoid basal cell carcinoma syndrome (7,8). PTCH proteins have a sterol-sensing domain (SSD) also found in several proteins that function in cholesterol homeostasis, such as HMGCR (3-hydroxy-3-methylglutaryl coenzyme A-reductase) and SCAP (sterol regulatory element-binding protein-cleavage activating protein). However, the role of the SSD in Patched proteins is not clear (9,10).

PTCH1 itself is a target of Hedgehog signaling (11), with elevated PTCH1 expression as a surrogate marker for Hedgehog pathway activation (12-14).

  1. Stone, D.M. et al. (1996) Nature 384, 129-134.
  2. Chen, Y. and Struhl, G. (1996) Cell 87, 553-563.
  3. Motoyama, J. et al. (1998) Nat. Genet. 18, 104-106.
  4. Smyth, I. et al. (1999) Hum. Mol. Genet. 8, 291-297.
  5. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
  6. McMahon, A.P. et al. (2003) Curr. Top. Dev. Biol. 53, 1-114.
  7. Hahn, H. et al. (1996) Cell 85, 841-851.
  8. Johnson, R.L. et al. (1996) Science 272, 1668-1671.
  9. Kuwabara, P.E. and Labouesse, M. (2002) Trends Genet. 18, 193-201.
  10. Chang, T.Y. et al. (2006) Annu. Rev. Cell Dev. Biol. 22, 129-157.
  11. Agren, M. et al. (2004) Gene 330, 101-114.
  12. Watkins, D.N. et al. (2003) Nature 422, 313-317.
  13. Berman, D.M. et al. (2003) Nature 425, 846-851.
  14. Karhadkar, S.S. et al. (2004) Nature 431, 707-712.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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