Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

PTCH2 (G1191) Antibody #2470

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Transfected Only 130 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

PTCH2 (G1191) Antibody detects transfected levels of PTCH2 protein. It does not recognize transfected levels of human PTCH1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region (predicted to be intracellular) surrounding residue Gly1191 of human PTCH2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transiently transfected with a construct expressing human PTCH2, using PTCH2 (G1191) Antibody.

Background

Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the receiving receptors for members of the Hedgehog family of proteins (1-4). In the absence of Hedgehog proteins, PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) so that the Hedgehog signaling pathway is in the off state (5,6). Deactivating mutations that impair the ability of PTCH1 to suppress Smo are frequently found in patients with nevoid basal cell carcinoma syndrome (7,8). PTCH proteins have a sterol-sensing domain (SSD) also found in several proteins that function in cholesterol homeostasis, such as HMGCR (3-hydroxy-3-methylglutaryl coenzyme A-reductase) and SCAP (sterol regulatory element-binding protein-cleavage activating protein). However, the role of the SSD in Patched proteins is not clear (9,10).

  1. Stone, D.M. et al. (1996) Nature 384, 129-134.
  2. Chen, Y. and Struhl, G. (1996) Cell 87, 553-563.
  3. Motoyama, J. et al. (1998) Nat. Genet. 18, 104-106.
  4. Smyth, I. et al. (1999) Hum. Mol. Genet. 8, 291-297.
  5. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
  6. McMahon, A.P. et al. (2003) Curr. Top. Dev. Biol. 53, 1-114.
  7. Hahn, H. et al. (1996) Cell 85, 841-851.
  8. Johnson, R.L. et al. (1996) Science 272, 1668-1671.
  9. Kuwabara, P.E. and Labouesse, M. (2002) Trends Genet. 18, 193-201.
  10. Chang, T.Y. et al. (2006) Annu. Rev. Cell Dev. Biol. 22, 129-157.

Application References

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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