Product Pathways - Protein Stability
PSA/KLK3 (D11E1) XP® Rabbit mAb #2475
PhosphoSitePlus® protein, site, and accession data: KLK3
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P | H | Endogenous | 29 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2475:
- IHC / Paraffin, Western Blotting
Specificity / Sensitivity
PSA/KLK3 (D11E1) XP® Rabbit mAb recognizes endogenous levels of total PSA/KLK3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val72 of human PSA/KLK3 protein.
Western Blotting
Western blot analysis of extracts from human sperm and LNCaP and DU 145 cells using PSA/KLK3 (D11E1) XP® Rabbit mAb. DU 145 cells are negative as expected.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded cell pellets, LNCaP (left) or DU 145 (right), using PSA/KLK3 (D11E1) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using PSA/KLK3 (D11E1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Background
Kallikrein 3 (KLK3), also known as Prostate Specific Antigen (PSA), is a member of the glandular kallikrein subfamily of serine proteases (1). It is produced by prostate epithelial cells and is secreted into prostatic ducts. Upon cleavage of 7 N-terminal amino acids (2), it is activated to liquefy semen in the seminal coagulum. Although PSA/KLK3 is produced in healthy individuals, investigators have found abnormally high levels in the blood of men with advanced prostate cancer (3,4).
- Watt, K.W. et al. (1986) Proc Natl Acad Sci U S A 83, 3166-70.
- Balk, S.P. et al. (2003) J Clin Oncol 21, 383-91.
- Balk, S.P. et al. (2003) J Clin Oncol 21, 383-91.
- Thompson, I.M. et al. (2004) N Engl J Med 350, 2239-46.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 5425 Normal Goat Serum
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 8059 SignalStain® DAB Substrate Kit
For Research Use Only. Not For Use In Diagnostic Procedures.
