Product Pathways - Tyrosine Kinase/ Adaptors
VEGF Receptor 2 (55B11) Rabbit mAb #2479
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-F IF-IC F | H M | Endogenous | 210, 230 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-F=Immunofluorescence (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
VEGF Receptor 2 (55B11) Rabbit Monoclonal Antibody detects endogenous levels of VEGF receptor 2 protein. This antibody does not cross-react with other family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein containing the carboxy-terminal 150 amino acid residues of human VEGF receptor 2.
Western Blotting
Western blot analysis of extracts from various cell lines and primary cell cultures, using VEGF Receptor 2 (55B11) Rabbit mAb. PAE/CKR cells overexpress chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor 2 intracellular domains (Rahimi, N. et al. [2000] J. Biol. Chem. 275, 16986-16992). PAE/VEGFR1 cells overexpress human VEGF receptor 1.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human astrocytoma, using VEGF Receptor 2 (55B11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded breast angiosarcoma, using VEGF Receptor 2 (55B11) Rabbit mAb (left). A serial section is stained for CD31 (PECAM-1), an endothelial cell marker (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HUVEC cells, using VEGF Receptor 2 (55B11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human renal adenocarcinoma, using VEGF Receptor 2 (55B11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HT-29 xenograft, using VEGF Receptor 2 (55B11) Rabbit mAb. Note staining of mouse blood vessels.
Flow Cytometry
Flow cytometric analysis of CKR/PAE cells, using VEGF Receptor 2 (55B11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent images of HUVEC cells untreated (left) or stimulated with Vascular Endothelial Growth Factor (VEGF) #9943 (right) and labeled with Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 (top, green) and VEGF Receptor 2 (55B11) Rabbit mAb (bottom, green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
IF-F
Confocal immunofluorescent analysis of mouse pancreas using VEGF Receptor 2 (55B11) Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).
- Meyer, M. et al. (1999) EMBO J 18, 363-74.
- Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
- Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
- Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
- Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
- Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
- Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
- Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.
Application References
- Antonescu, C.R. et al. (2009) Cancer Res , . Applications: IF-IC (In Cells) IHC-P (paraffin) Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2904 VEGF Receptor 2 Control Proteins
- 3770 Phospho-VEGF Receptor 2 (Tyr1175) (D5B11) XP™ Rabbit mAb
- 2478 Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb
- 3627 VEGF Receptor 2 (55B11) Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 3628 VEGF Receptor 2 (55B11) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 3817 Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) XP™ Rabbit mAb
- 4991 Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb
- 2477 Phospho-VEGF Receptor 2 (Tyr1212) (11A3) Rabbit mAb
- 2471 Phospho-VEGF Receptor 2 (Tyr951) Antibody
- 2472 VEGF Receptor 2 Antibody
- 2474 Phospho-VEGF Receptor 2 (Tyr996) Antibody
- 2476 Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb
- 2893 VEGF Receptor 1 Antibody
- 2638 VEGF Receptor 3 (C28G5) Rabbit mAb
- 2485 VEGF Receptor 3 Antibody
- 2463 VEGF-B Antibody
- 2445 VEGF-C Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and U.S.S.N. 11/476,277) from Epitomics, Inc.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
