Product Pathways - Tyrosine Kinase/ Adaptors
VEGF Receptor 2 (55B11) Rabbit mAb #2479
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M | Endogenous | 210, 230 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
VEGF Receptor 2 (55B11) Rabbit Monoclonal Antibody detects endogenous levels of VEGF receptor 2 protein. This antibody does not cross-react with other family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST-fusion protein containing the carboxy-terminal 150 amino acid residues of human VEGF receptor 2.
Western Blotting
Western blot analysis of extracts from various cell lines and primary cell cultures, using VEGF Receptor 2 (55B11) Rabbit mAb. PAE/CKR cells overexpress chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor 2 intracellular domains (Rahimi, N. et al. [2000] J. Biol. Chem. 275, 16986-16992). PAE/VEGFR1 cells overexpress human VEGF receptor 1.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human astrocytoma, using VEGF Receptor 2 (55B11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded breast angiosarcoma, using VEGF Receptor 2 (55B11) Rabbit mAb (left). A serial section is stained for CD31 (PECAM-1), an endothelial cell marker (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HUVEC cells, using VEGF Receptor 2 (55B11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human renal adenocarcinoma, using VEGF Receptor 2 (55B11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HT-29 xenograft, using VEGF Receptor 2 (55B11) Rabbit mAb. Note staining of mouse blood vessels.
Flow Cytometry
Flow cytometric analysis of CKR/PAE cells, using VEGF Receptor 2 (55B11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent images of HUVEC cells untreated (left) or stimulated with Vascular Endothelial Growth Factor (VEGF) #9943 (right) and labeled with Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 (top, green) and VEGF Receptor 2 (55B11) Rabbit mAb (bottom, green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor transducing VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).
- Meyer, M. et al. (1999) EMBO J. 18, 363-374.
- Dougher-Vermazen, M. et al. (1994) Biochem. Biophys. Res. Commun. 205, 728-738.
- Kroll, J. and Waltenberger, J. (1997) J. Biol. Chem. 272, 32521-32527.
- Takahashi, T. et al. (2001) EMBO J. 20, 2768-2778.
- Holmqvist, K. et al. (2004) J. Biol. Chem. 279, 22267-22275.
- Karkkainen, M.J. and Petrova, T. (2000) Oncogene 19, 5598-5605.
- Rahimi, N. et al. (2000) J. Biol. Chem. 275, 16986-16992.
- Claesson-Welsh, L. (2003) Biochem. Soc. Trans. 31, 20-24.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2471 Phospho-VEGF Receptor 2 (Tyr951) Antibody
- 2472 VEGF Receptor 2 Antibody
- 2474 Phospho-VEGF Receptor 2 (Tyr996) Antibody
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- 2478 Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb
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- 2476 Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb
- 4991 Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb
- 2485 VEGF Receptor 3 Antibody
- 2638 VEGF Receptor 3 (C28G5) Rabbit mAb
- 2445 VEGF-C Antibody
- 2463 VEGF-B Antibody
- 9943 Vascular Endothelial Growth Factor (VEGF)
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
