Product Pathways - Translational Control
eIF5 Antibody #2480
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R Mk | Endogenous | 58 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
eIF5 Antibody detects endogenous levels of total eIF5 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from a sequence surrounding Val417 of human eIF5. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines using eIF5 Antibody.
IF-IC
Confocal immunofluorescent analysis of MCF-7 cells using eIF5 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Eukaryotic translation initiation factor 5 (eIF5) is crucial for the assembly of translation initiation complex and plays an important role in protein synthesis (1). eIF5 interacts with the 43S initiation complex to stimulate hydrolysis of GTP bound to eIF2 (1-3). Studies suggest that eIF5 functions as the GTPase-activating protein (GAP) in the hydrolysis of GTP-bound eIF2 (4,5). This hydrolysis leads to the release of initiation factors from the 40S ribosomal subunit, which is a necessary step in the formation of the 80S initiation complex (1).
- Das, S. and Maitra, U. (2001) Prog. Nucleic Acid Res. Mol. Biol. 70, 207-231.
- Chakrabarti, A. and Maitra, U. (1991) J. Biol. Chem. 266, 14039-14045.
- Asano, K. et al. (2000) Genes Dev. 14, 2534-2546.
- Das, S. et al. (2001) J. Biol. Chem. 276, 6720-6726.
- Paulin, F.E. et al. (2001) Curr. Biol. 11, 55-59.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
