Cell Signaling Technology

Product Pathways - Translational Control

eIF5 Antibody #2480

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 58 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

eIF5 Antibody detects endogenous levels of total eIF5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence surrounding Val417 of human eIF5. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using eIF5 Antibody.

Background

Eukaryotic translation initiation factor 5 (eIF5) is crucial for the assembly of translation initiation complex and plays an important role in protein synthesis (1). eIF5 interacts with the 43S initiation complex to stimulate hydrolysis of GTP bound to eIF2 (1-3). Studies suggest that eIF5 functions as the GTPase-activating protein (GAP) in the hydrolysis of GTP-bound eIF2 (4,5). This hydrolysis leads to the release of initiation factors from the 40S ribosomal subunit, which is a necessary step in the formation of the 80S initiation complex (1).

  1. Das, S. and Maitra, U. (2001) Prog. Nucleic Acid Res. Mol. Biol. 70, 207-231.
  2. Chakrabarti, A. and Maitra, U. (1991) J. Biol. Chem. 266, 14039-14045.
  3. Asano, K. et al. (2000) Genes Dev. 14, 2534-2546.
  4. Das, S. et al. (2001) J. Biol. Chem. 276, 6720-6726.
  5. Paulin, F.E. et al. (2001) Curr. Biol. 11, 55-59.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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