Cell Signaling Technology

Product Pathways - Development

DLL3 (G93) Antibody #2483

Applications Reactivity Sensitivity MW (kDa) Source
W IP R (M) Transfected Only 65 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DLL3 (G93) Antibody detects transfected levels of DLL3. It does not recognize transfected levels of rat DLL1 and human DLL4.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding residue Gly93 of mouse DLL3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of COS cell extracts, untransfected or transiently transfected with a construct expressing rat DLL3 protein, using DLL3 (G93) Antibody.

Background

Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6).

Mutations in DLL3 cause Spondylocostal dysostoses (SCD), a diverse group of disorders of axial skeletal malformation (7-10).

  1. Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860-2868.
  2. Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320-328.
  3. Chiba, S. (2006) Stem Cells 24, 2437-2447.
  4. Bland, C.E. et al. (2003) J. Biol. Chem. 278, 13607-13610.
  5. Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643.
  6. LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437.
  7. Whittock, N.V. et al. (2004) Clin. Genet. 66, 67-72.
  8. Turnpenny, P.D. et al. (2003) J. Med. Genet. 40, 333-339.
  9. Bulman, M.P. et al. (2000) Nat. Genet. 24, 438-441.
  10. Bonafé, L. et al. (2003) Clin. Genet. 64, 28-35.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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