Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

VEGF Receptor 3 Antibody #2485

Applications Reactivity Sensitivity MW (kDa) Source
W H Transfected Only 195 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

VEGF Receptor 3 Antibody detects transfected levels of VEGFR-3. This antibody does not cross-react with VEGFR-1 and -2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg961 of human VEGFR-3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of cell lysates from COS cells, either untransfected or overexpressing VEGFR-3, using VEGF Receptor 3 Antibody.

Background

Vascular endothelial growth factor receptor 3 (VEGFR3) is a 195 kDa membrane receptor tyrosine kinase. VEGF receptors are characterized by the presence of seven extracellular immunoglobulin (Ig)-like domains followed by a membrane-spanning domain and a conserved intracellular tyrosine kinase domain (1). VEGF receptor 3 expression is largely restricted to adult lymphatic endothelium and is thought to control lymphangiogenesis (1,2). Binding of VEGF-C/VEGF-D to VEGFR3 results in transphosphorylation of tyrosine residues in its intracellular domain, recruitment of signaling molecules and activation of ERK1/2 and Akt signaling cascades (1,3).

  1. Robinson, C.J. and Stringer, S.E. (2001) J. Cell Sci.114, 853-865.
  2. Valtola, R. et al. (1999) Am. J. Pathol. 154, 1381-1390.
  3. Saharinen, P. and Petrova, T.V. (2004) Ann. N.Y. Acad. Sci. 1014, 76-87.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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