Product Pathways - Tyrosine Kinase/ Adaptors
VEGF Receptor 3 Antibody #2485
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Transfected Only | 195 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
VEGF Receptor 3 Antibody detects transfected levels of VEGFR-3. This antibody does not cross-react with VEGFR-1 and -2.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Arg961 of human VEGFR-3. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of cell lysates from COS cells, either untransfected or overexpressing VEGFR-3, using VEGF Receptor 3 Antibody.
Background
Vascular endothelial growth factor receptor 3 (VEGFR3) is a 195 kDa membrane receptor tyrosine kinase. VEGF receptors are characterized by the presence of seven extracellular immunoglobulin (Ig)-like domains followed by a membrane-spanning domain and a conserved intracellular tyrosine kinase domain (1). VEGF Receptor 3 expression is largely restricted to adult lymphatic endothelium and is thought to control lymphangiogenesis (1,2). Binding of VEGF-C/VEGF-D to VEGFR3 results in transphosphorylation of tyrosine residues in its intracellular domain, recruitment of signaling molecules and activation of ERK1/2 and Akt signaling cascades (1,3).
- Robinson, C.J. and Stringer, S.E. (2001) J. Cell Sci.114, 853-865.
- Valtola, R. et al. (1999) Am. J. Pathol. 154, 1381-1390.
- Saharinen, P. and Petrova, T.V. (2004) Ann. N.Y. Acad. Sci. 1014, 76-87.
Application References
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Companion Products
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- 2471 Phospho-VEGF Receptor 2 (Tyr951) Antibody
- 2472 VEGF Receptor 2 Antibody
- 2474 Phospho-VEGF Receptor 2 (Tyr996) Antibody
- 2476 Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb
- 2477 Phospho-VEGF Receptor 2 (Tyr1212) (11A3) Rabbit mAb
- 2478 Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb
- 2479 VEGF Receptor 2 (55B11) Rabbit mAb
- 4991 Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb
- 2463 VEGF-B Antibody
- 2638 VEGF Receptor 3 (C28G5) Rabbit mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
