Cell Signaling Technology

Product Pathways - Translational Control

eIF4A1 Antibody #2490

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M R Mk Endogenous 48 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

eIF4A1 Antibody detects endogenous levels of total eIF4A1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence around Gly12 of human eIF4A1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using eIF4A1 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using eIF4A1 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

A variety of factors contribute to the important biological event of initiation of translation. The eIF4F complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signalling in a translation-independent manner in Drosophila (4,5).

  1. Rogers, G.W. et al. (2001) J. Biol. Chem. 276, 12598-12608.
  2. Rogers, G.W. et al. (1999) J. Biol. Chem. 274, 12236-12244.
  3. Svitkin, Y.V. et al. (2001) RNA 7, 382-394.
  4. Li, J. and Li, W.X. (2006) Nat. Cell Biol. 8, 1407-1414.
  5. Affolter, M. and Pyrowolakis, G. (2006) Nat. Cell Biol. 8, 1319-1321.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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