Product Pathways - Glucose Metabolism
Phospho-IRS-1 (Ser302) (34C7) Rabbit mAb #2491
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W | H M | Transfected Only | 180 | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-IRS-1 (Ser302) (37C7) Rabbit mAb detects transfected levels of IRS-1 only when phosphorylated at Ser302.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser302 of mouse IRS-1 (equivalent to Ser307 of human IRS-1).
Western Blotting
Western blot analysis of cell extracts from CHO cells overexpressing insulin receptor and IRS-1, untreated or treated with insulin, using Phospho-IRS-1 (Ser302) (34C7) Rabbit mAb (upper and middle) or IRS-1 Antibody #2382 (lower). The middle blot was treated with CIP before antibody probing.
Background
Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain containing proteins that mediate the metabolic and growth promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).
- Sun, X.J. et al. (1991) Nature 352, 73-77.
- Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672.
- Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430.
- Wang, L.M. et al. (1993) Science 261, 1591-1594.
- Rui, L. et al. (1997) J. Clin. Invest. 107, 181-189.
- Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121.
- Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447.
- Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645.
- De Fea, K. and Ruth, R.A. (1997) Biochemistry 36, 12939-12947.
- Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.
Application References
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Companion Products
- 2384 Phospho-IRS-1 (Ser302) Antibody
- 2381 Phospho-IRS-1 (Ser307) Antibody
- 2386 Phospho-IRS-1 (Ser612) Antibody
- 3193 Phospho-IRS-1 (Ser612) (L7B8) Mouse mAb
- 2388 Phospho-IRS-1 (Ser636/639) Antibody
- 2389 Phospho-IRS-1 (Ser789) Antibody
- 2385 Phospho-IRS-1 (Ser1101) Antibody
- 2382 IRS-1 Antibody
- 3194 IRS-1 (L3D12) Mouse mAb
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- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
