Cell Signaling Technology

Product Pathways - NF-kB Signaling

MALT1 Antibody #2494

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 90 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MALT1 Antibody detects endogenous levels of total MALT1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of mouse and rat MALT1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MALT1 Antibody.

Background

Mucosa-associated lymphoid tissue translocation gene 1 (MALT1) is a paracaspase that is a critical mediator of T-cell receptor activation of NF-κB and may contribute to the progression of MALT lymphomas (1-4). It contains two immunoglobulin-like domains, an amino-terminal death domain and a carboxy-terminal caspase-like domain. Association of MALT1 with Bcl-10 and CARD11/Carma1 leads to activation of IKK and subsequent stimulation of NF-κB, resulting in increased proliferation and inhibition of apoptosis (5,6). A common translocation in MALT B-cell non-Hodgkin lymphomas t(11;18)(q21;q21) results in the fusion of the amino terminus of API2 (c-IAP2), a member of the inhibitor of apoptosis protein family, to the carboxy terminus of MALT1 (1,2). The API2-MALT1 fusion protein likely leads to deregulation of NF-κB, contributing to increased oncogenic potential (7).

  1. Akagi, T. et al. (1999) Oncogene 18, 5785-5794.
  2. Uren, A.G. et al. (2000) Mol. Cell 6, 961-967.
  3. Ruland, J. et al. (2003) Immunity 19, 749-758.
  4. Nakagawa, M. et al. (2006) Leukemia 20, 929-936.
  5. Che, T. et al. (2004) J. Biol. Chem. 279, 15870-15876.
  6. Lucas, P.C. et al. (2001) J. Biol. Chem. 276, 19012-19019.
  7. Ho, L. et al. (2005) Blood 105, 2891-2899.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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