Product Pathways - NF-kappaB Signaling
MALT1 Antibody #2494
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 90 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
MALT1 Antibody detects endogenous levels of total MALT1 protein.
Source / Purification
Polyclonal antibodies are prepared by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to the carboxy terminus of mouse/rat MALT1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 786-0, Ramos, RL and A20 cell lines, using MALT1 Antibody.
Background
Mucosa-associated lymphoid tissue translocation gene 1 (MALT1) is a paracaspase that is a critical mediator of T-cell receptor activation of NF-κB and may contribute to the progression of MALT lymphomas (1-4). It contains two immunoglobulin-like domains, an amino-terminal death domain and a carboxy-terminal caspase-like domain. Assocation of MALT1 with Bcl-10 and CARD11/Carma1 leads to activation of IKK and subsequent stimulation of NF-κB, resulting in increased proliferation and inhibition of apoptosis (5,6). A common translocation in MALT B-cell non-Hodgkin lymophomas t(11;18)(q21;q21) results in the fusion of the amino terminus of API2 (c-IAP2), a member of the inhibitor of apoptosis protein family, to the carboxy terminus of MALT1 (1,2). The API2-MALT1 fusion protein likely leads to deregulation of NF-κB, contributing to increased oncogenic potential (7,8).
- Akagi, T. et al. (1999) Oncogene 18, 5785-5794.
- Uren, A.G. et al. (2000) Mol. Cell 6, 961-967.
- Ruland, J. et al. (2003) Immunity 19, 749-758.
- Nakagawa, M. et al. (2006) Leukemia 20, 929-936.
- Che, T. et al. (2004) J. Biol. Chem. 279, 15870-15876.
- Lucas, P.C. et al. (2001) J. Biol. Chem. 276, 19012-19019.
- Ho, L. et al. (2005) Blood 105, 2891-2899.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
