Product Pathways - Translational Control
eIF4G Antibody #2498
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P IF-IC F | H M R Mk | Endogenous | 220 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
eIF4G Antibody detects endogenous levels of total eIF4G protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from a sequence of human eIF4G. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, using eIF4G Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using eIF4G Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic localization, using eIF4G Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using eIF4G Antibody in the presence of control peptide (left) or eIF4G blocking peptide #1003 (right).
Flow Cytometry
Flow cytometric analysis of HeLa cells, using eIF4G Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells, using eIF4G Antibody (green, left) compared to an isotype control (right). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5' m7GTP mRNA cap and function to unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A (4) and serves as an adaptor molecule in the eIF4 complex. Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). Two distinct eIF4G species have been identified; eIF4GI and eIF4GII share 46% identity in their amino acid sequences and are functionally related homologs (7).
- Yan, R. and Rhoads, R.E. (1995) Genomics 26, 394-398.
- Morley, S.J. et al. (1997) RNA 3, 1085-1104.
- Haghighat, A. and Sonenberg, N. (1997) J. Biol. Chem. 272, 21677-21680.
- De Gregorio, E. et al. (1998) RNA 4, 828-836.
- Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
- Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
- Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
