Product Pathways - Cytoskeletal Signaling
Vav Antibody #2502
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP F | H M R | Endogenous | 95 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Vav Antibody detectendogenous levels of total Vav protein. The antibody does not cross-react with Vav2, Vav3 or CelVav.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Tyr174 of human Vav. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat and THP1 cells, using Vav Antibody.
IP
Immunoprecipitation of Jurkat and THP1 cell extracts, using Vav Antibody, followed by Western blot analysis using the same antibody.
Flow Cytometry
Flow cytometric analysis of untreated Jurkat cells, using Vav antibody (blue) compared to a nonspecific negative control antibody (red).
Background
Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3).
- Hornstein, I. et al. (2004) Cell. Signal. 16, 1-11.
- Crespo, P. et al. (1997) Nature 385, 169-172.
- Han, J. et al. (1997) Mol. Cell. Biol. 17, 1346-1353.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
