Product Pathways - Cytoskeletal Signaling
Vav1 Antibody #2502
|W F||H M R||Endogenous||95||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Vav Antibody detects endogenous levels of total Vav1 protein. The antibody does not cross-react with Vav2, Vav3 or CelVav.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr174 of human Vav. Antibodies are purified by protein A and peptide affinity chromatography.
Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3).
- Hornstein, I. et al. (2004) Cell. Signal. 16, 1-11.
- Crespo, P. et al. (1997) Nature 385, 169-172.
- Han, J. et al. (1997) Mol. Cell. Biol. 17, 1346-1353.
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For Research Use Only. Not For Use In Diagnostic Procedures.