Cell Signaling Technology

Product Pathways - Neuroscience

PSD95 Antibody #2507

Applications Reactivity MW (kDa) Source
W IP IF-F M R (H) 95 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

PSD95 Antibody detects endogenous levels of total PSD95 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues of human PSD95. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain, using PSD95 Antibody.

IF-F

IF-F

Confocal immunofluorescent image of rat retina labeled with PSD95 Antibody (red) and Akt (5G3) Mouse mAb #2966 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

Postsynaptic Density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. These family members consist of an N-terminal variable segment followed by three PDZ domains, a SH3 domain and an inactive guanylate kinase (GK) domain. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1-2). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (3). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (4). Mutations in PSD95 are associated with autism (5).

  1. Cao, J. et al. (2005) J. Cell Biol 168, 117-26.
  2. Chetkovich, D.M. et al. (2002) J. Neurosci. 22, 6415-25.
  3. Cai, C. et al. (2006) J. Biol. Chem. 281, 4267-73.
  4. Yao, W.D. et al. (2004) Neuron 41, 625-38.
  5. Cline, H. (2005) Curr. Biol. 15, R203-5.

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