Product Pathways - Neuroscience
TrkA (14G6) Rabbit mAb #2508
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IHC-P | H | 140 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
TrkA (14G6) Rabbit mAb detects endogenous levels of total TrkA protein. This antibody does not cross-react with TrkB.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) surrounding Arg220 of human TrkA.
Western Blotting
Western blot analysis of extracts from NIH/3T3, NIH/3T3-TrkA, NIH/3T3-TrkB and NIH/3T3-TrkC and K562 cells using TrkA (14G6) Rabbit mAb (upper) TrkB (80G2) Rabbit mAb Antibody #4607 (middle) and PLCγ1 Antibody #2822 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast using TrkA (14G6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Trk A (14G6) Rabbit mAb #2508 in the presence of control peptide (left) or Trk A Blocking Peptide #1435 (right).
Background
The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, these family members are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4 and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimera) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies (breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia) have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1).
- Pierotti, M.A. and Greco, A. (2006) Cancer Lett. 232, 90-98.
- Segal, R.A. and Greenberg, M.E. (1996) Annu. Rev. Neurosci. 19, 463-489.
- Stephens, R.M. et al. (1994) Neuron 12, 691-705.
- Obermeier, A. et al. (1993) EMBO J. 12, 933-941.
- Obermeier, A. et al. (1994) EMBO J. 13, 1585-1590.
- Yao, R. and Cooper, G.M. (1995) Science 267, 2003-2006.
Application References
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Companion Products
- 9141 Phospho-TrkA (Tyr490) Antibody
- 2505 TrkA Antibody
- 7422 TrkA Kinase
- 4606 TrkB Antibody
- 2431 Phospho-Shc (Tyr317) Antibody
- 2432 Shc Antibody
- 3965 Ras Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 1435 TrkA Blocking Peptide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
