Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

AP-2β Antibody #2509

Applications Reactivity MW (kDa) Source
W IP IF-IC H M R 49 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

AP-2β Antibody detects endogenous levels of total AP-2β protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human AP-2β. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using AP-2β Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of SH-SY5Y cells labeled with AP-2β Antibody (green, left) compared to a nonspecific negative control antibody (right). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Background

The sequence-specific transcription factor activator protein 2β (AP-2β) is required for normal kidney development (1). AP-2β knockout mice die 1-2 days after birth due to polycystic kidney disease (1). Massive apoptosis occured during kidney deveopment at the end of embryogenesis in these mice (1). Overexpressed AP-2β has been to found to suppress c-myc-induced apoptosis, indicating a role of this transcription factor in cell survival (1). In addition, overexpression of AP-2β is shown to be related to impaired insulin signaling in adipocytes, and is therefore proposed to be a candidate gene that may relate to obesity (2).

  1. Moser, M. et al. (1997) Genes Dev. 11, 1938-1148.
  2. Tao, Y. et al. (2006) Endocrinology 147, 1685-1696.

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