Product Pathways - Nuclear Receptor Signaling
Phospho-Estrogen Receptor α (Ser118) Antibody #2515
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 66 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Protocols
- 2515:
- Western Blotting
Specificity / Sensitivity
Phospho-Estrogen Receptor alpha (Ser118) Antibody detects endogenous levels of estrogen receptor alpha only when phosphorylated at Ser118. The antibody does not cross-react with the phosphorylated ER isoform beta.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser118 of human ER alpha. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from MCF-7 cells, untreated or treated with EGF (100 ng/ml) and E2 (100 nM) for 30 minutes, using Phospho-Estrogen Receptor alpha (Ser118) Antibody (upper) or control ER alpha antibody (lower).
Western Blotting
Western blot analysis of extracts from COS-1 cells expressing wild-type or mutant ER alpha (S106A, S118A and S167A), treated with EGF (100 ng/ml) and E2 (10 -7 M) for 30 minutes, using Phospho-Estrogen Receptor alpha (Ser118) Antibody (upper) or control ER alpha antibody (lower).
Background
Estrogen receptorα (ER α), a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD) (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER α regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation provides an important mechanism to regulate ER α activity (3,4). ER α is phosphorylated on multiple sites (5). Serines 104, 106, 118 and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ER α activity. Ser118 may be the substrate of the transcription regulatory kinase cdK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients (4).
- Mangelsdorf, D.J. et al. (1995) Cell 83, 835-839.
- Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev. 14, 121-141.
- Chen, D. et al. (1999) Mol. Cell. Biol. 19, 1002-1015.
- Campbell, R.A. et al. (2001) J. Biol. Chem. 276, 9817-9824.
- Chen, D. et al. (2000) Mol. Cell 6, 127-137.
- Joel, P.B. et al. (1998) Mol. Cell. Biol. 18, 1978-1984.
Application References
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Companion Products
- 2512 Estrogen Receptor α (62A3) Mouse mAb
- 2511 Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb
- 2517 Phospho-Estrogen Receptor α (Ser104/106) Antibody
- 2514 Phospho-Estrogen Receptor α (Ser167) Antibody
- 9924 Phospho-Estrogen Receptor α Antibody Sampler Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
