Cell Signaling Technology

Product Pathways - Development

SUFU (C81H7) Rabbit mAb #2522

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Mk Endogenous 54 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SUFU (C81H7) Rabbit mAb detects endogenous level of total SUFU protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala340 of human SUFU.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from HeLa, COS and NIH/3T3 cells using SUFU (C81H7) Rabbit mAb.

Background

SUFU (Suppressor of Fused) was identified in Drosophila as a suppressor of the Fused (Fu) kinase that is essential for Hedgehog signaling during embryonic pattern formation (1). SUFU suppresses Hedgehog signaling by regulating the localization of the transcription factors Gli and Ci (2,3). In Drosophila, SUFU may also positively regulate Hedgehog signaling depending on SUFU protein levels and Hedgehog signal intensity (4). SUFU may function as a tumor suppressor as inactivation and loss of heterozygosity of SUFU is associated with human rhabdomyosarcomas and medulloblastomas (5,6). Deletion of SUFU in mice results in embryonic lethality, while heterozygotes exhibit developmental defects characteristic of basal cell nevus syndrome. This aberrant developmental pathway is attributed to ligand-independent activation of Hedgehog signaling (7). GSK-3β binds and phosphorylates SUFU in vitro and additional information predicts that GSK-3β may positively regulate Hedgehog signaling through modification of SUFU (8).

  1. Pham, A. et al. (1995) Genetics 140, 587-598.
  2. Barnfield, P.C. et al. (2005) Differentiation 73, 397-405.
  3. Méthot, N. and Basler, K. (2000) Development 127, 4001-4010.
  4. Dussillol-Godar, F. et al. (2006) Dev. Biol. 291, 53-66.
  5. Tostar, U. et al. (2006) J. Pathol. 208, 17-25.
  6. Taylor, M.D. et al. (2002) Nat. Genet. 31, 306-310.
  7. Svärd, J. et al. (2006) Dev. Cell. 10, 187-197.
  8. Takenaka, K. et al. (2007) Biochem. Biophys. Res. Commun. 353, 501-508.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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