Product Pathways - Jak/Stat Pathway
Acetyl-Stat3 (Lys685) Antibody #2523
|2523L||300 µl (30 western blots)||---||In Stock||---|
|2523S||100 µl (10 western blots)||---||In Stock||---|
|2523||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human||Transfected Only||79, 86||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Mouse, Rat.
Specificity / Sensitivity
Acetyl-Stat3 (Lys685) Antibody detects transfected Stat3 when acetylated at lysine 685.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding lysine 685 of Stat3. Antibodies were purified by protein A and peptide affinity chromatography.
The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
In addition to phosphorylation, Stat3 can be modified by acetylation. Stat3 is acetylated at Lys685 by p300/CREB-binding protein (CBP) which can stimulate DNA binding and transactivation activity (11,12).
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- Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
- Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
- Yuan, Z.L. et al. (2005) Science 307, 269-73.
- Wang, R. et al. (2005) J. Biol. Chem. 280, 11528-34.
- Nie, Y. et al. (2009) Nat Cell Biol 11, 492-500. Applications: Western Blotting.
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