Product Pathways - Jak/Stat Pathway
Acetyl-Stat3 (Lys685) Antibody #2523
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H (M) (R) | Transfected Only | 79, 86 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Acetyl-Stat3 (Lys685) Antibody detects transfected Stat3 when acetylated at lysine 685.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic acetylated peptide (KLH-coupled) corresponding to residues surrounding lysine 685 of Stat3. Antibodies were purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells transfected with Stat3, CBP or both, using Acetyl-Stat3 (Lys685) Antibody (top) or total Stat3 Antibody #9139.
Background
The Stat3 transcription factor is an important signaling molecule for many cytokines and growth-factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
In addition to phosphorylation, Stat3 can be modified by acetylation. Stat3 is acetylated at Lys885 by p300/CREB-binding protein (CBP) which can stimulate DNA binding and transactivation activity (9,10).
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- Yokogami, K. et al. (2000) Curr. Biol. 10, 47-50.
- Biethahn, S. et al. (1999) Exp. Hematol. 27, 885-894.
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- Wang, R. et al. (2005) J. Biol. Chem. 280, 11528-34.
Application References
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Companion Products
- 9131 Phospho-Stat3 (Tyr705) Antibody
- 9132 Stat3 Antibody
- 4904 Stat3 (79D7) Rabbit mAb
- 9139 Stat3 (124H6) Mouse mAb
- 9134 Phospho-Stat3 (Ser727) Antibody
- 9136 Phospho-Stat3 (Ser727) (6E4) Mouse mAb
- 9138 Phospho-Stat3 (Tyr705) (3E2) Mouse mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
