Product Pathways - Adhesion
Phospho-Paxillin (Tyr118) Antibody #2541
PhosphoSitePlus® protein, site, and accession data: PXN iso2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R Mk Pg | Endogenous | 68 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Phospho-Paxillin (Tyr118) Antibody detects endogenous levels of paxillin only when phosphorylated at tyrosine 118. The antibody does not cross-react with other tyrosine phosphorylated proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr118 of human paxillin. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of untreated and lambda-phosphatase treated COS cells, using Phospho-Paxillin (Tyr118) Antibody (left) and Paxillin Antibody #2542 (right).
Western Blotting
Western blot analysis of extracts from various cell lines using Phospho-Paxillin (Tyr118) Antibody.
IF-IC
Confocal immunofluorescent analysis of Hela cells -/+ lamda phosphatse labeled with Phospho-Paxillin (Tyr118) Antibody (green). Note the robust signal in the untreated cells (left) versus the lambda phosphatse treated cells (right).Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). Red = phalloidin555.
Background
Paxillin is a multidomain protein that localizes primarily to focal adhesion sites in the extracellular matrix (1). Paxillin is one of the key components of integrin signaling, and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118 (3,4). Phospho-FAK (Tyr118) may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).
- Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140.
- Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.
- Bellis, S. L. et al. (1995) J. Biol. Chem. 270, 17437-17441.
- Bellis, S. L. et al. (1997) Biochem. J. 325, 375-381.
- Schaller, M.D. and Parsons, J.T. (1995) Mol. Cell. Biol. 15, 2635-2645.
Application References
- Zaytseva, Y.Y. et al. (2012) Cancer Res 72, 1504-17. Applications: IF-IC (In Cells) Western Blotting
- Biswas, K. et al. (2013) J Biol Chem 288, 2325-39. Applications: IF-IC (In Cells)
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Companion Products
- 2101 Phospho-Src Family (Tyr416) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2542 Paxillin Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.