Product Pathways - Adhesion
Paxillin Antibody #2542
PhosphoSitePlus® protein, site, and accession data: PXN iso2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk B Pg | Endogenous | 68 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2542:
- Western Blotting
Specificity / Sensitivity
Paxillin Antibody detects endogenous levels of total paxillin protein. The antibody does not cross-react with related proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human paxillin. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, using Paxillin Antibody.
Background
Paxillin is a multidomain protein that localizes primarily to focal adhesion sites in the extracellular matrix (1). Paxillin is one of the key components of integrin signaling, and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118 (3,4). Phospho-FAK (Tyr118) may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).
- Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140.
- Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.
- Bellis, S. L. et al. (1995) J. Biol. Chem. 270, 17437-17441.
- Bellis, S. L. et al. (1997) Biochem. J. 325, 375-381.
- Schaller, M.D. and Parsons, J.T. (1995) Mol. Cell. Biol. 15, 2635-2645.
Application References
- Zaytseva, Y.Y. et al. (2012) Cancer Res 72, 1504-17. Applications: Western Blotting
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Companion Products
- 2541 Phospho-Paxillin (Tyr118) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
