Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-cdc2 (Thr14) Antibody #2543

Applications Reactivity Sensitivity MW (kDa) Source
W H Hm Mk (M) (R) (Dm) (X) (B) Endogenous 34 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  Dm=D. melanogaster  X=Xenopus  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-cdc2 (Thr14) Antibody detects endogenous levels of cdc2 (CDK1) only when phosphorylated at Thr14. Based on sequence similarity, the antibody may cross-react with CDK2 and CDK3.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr14 of human cdc2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from hydroxyurea-treated HT29 cells, aphidicolin-treated HeLa cells, and asynchronous and mitotic CHO cells using Phospho-cdc2 (Thr14) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from asynchronous and mitotic HeLa cells using Phospho-cdc2 (Thr14) Antibody.

Background

The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).

  1. Atherton-Fessler, S. et al. (1994) Mol. Biol. Cell. 5, 989-1001.
  2. Norbury, C. et al. (1991) EMBO. J. 10, 3321-3329.
  3. McGowan, C.H. and Russell, P. (1993) EMBO J. 12, 75-85.
  4. Wells, N.J. et al. (1999) J. Cell. Sci. 112, 3361-3371.
  5. Hunter, T. (1995) Cell 80, 225-236.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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