Cell Signaling Technology

Product Pathways - Chromatin Regulation

HDAC2 Antibody (IP Preferred) #2545

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M Mk Endogenous 60 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

HDAC2 Antibody (IP Preferred) detects endogenous levels of total HDAC2 protein and is the preferred antibody for immunoprecipitation. The antibody does not cross-react with other HDAC proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of the human HDAC2 protein. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and DLD-1 cells using HDAC2 Antibody (IP Preferred).

IP

IP

Immunoprecipitation/Western blot analysis of extracts from 293 cells. Lane 1 contains lysate input (5%), lane 2 was immunoprecipitated with HDAC2 Antibody (IP Preferred) and lane 3 was immunoprecipitated with non-specific rabbit IgG. Western blot analysis was performed using HDAC2 Antibody (IP Preferred).

Background

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing trans factors increased accessibility to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate non-histone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I (HDACs 1, 2, 3 and 8) proteins are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9 and 10) are related to yeast Hda1-like proteins and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

HDAC1 and HDAC2 are highly homologous and are involved in histone deacetylation, chromatin remodeling and transcriptional repression (8-10). Both proteins are found together in numerous complexes including the nucleosome remodeling and deacetylation complex (NuRD), MeCP1, and the mSin3A corepressor complex.

  1. Marmorstein, R. et al. (2001) Cell. Mol. Life Sci. 58, 693-703.
  2. Gregory, P.D. et al. (2001) Exp. Cell Res. 265, 195-202.
  3. Liu, Y. et al. (2000) Mol. Cell. Biol. 20, 5540-5543.
  4. Cress, S.D. and Seto, E. (2000) J. Cell. Physiol. 184, 1-16.
  5. Gray, S.G. and Ekstrom, T.J. (2001) Exp. Cell Res. 262, 75-83.
  6. Thiagalingam, S. et al. (2003) Ann. N. Y. Acad. Sci. 983, 84-100.
  7. Viguishin, D.M. and Coombes, R.C. (2004) Curr. Cancer Drug Targets 4, 205-218.
  8. Zhang, Y. et al. (1999) Genes Dev. 13, 1924-1935.
  9. Ng, H.H. et al. (1999) Nat. Genet. 23, 58-61.
  10. Zhang, Y. et al. (1997) Cell 89, 357-364.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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