Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

CDK2 (78B2) Rabbit mAb #2546

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP F H M R Mk Endogenous 33 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CDK2 (78B2) Rabbit mAb detects endogenous levels of total CDK2 protein. The antibody does not cross-react with other CDKs.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of human CDK2 .

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using CDK2 (78B2) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, using CDK2 (78B2) Rabbit mAb versus propidium iodide (DNA content).

Background

Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5).

  1. Morgan, D.O. (1995) Nature 374, 131-134.
  2. Poon, R.Y. et al. (1996) J. Biol. Chem. 271, 13283-13291.
  3. Gu, Y. et al. (1992) EMBO J. 11, 3995-4005.
  4. Fesquet, D. et al. (1993) EMBO J. 12, 3111-3121.
  5. Morgan, D.O. (1997) Annu. Rev. Cell Dev. Biol. 13, 261-291.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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