Product Pathways - Related Products
Tat (8C2) Mouse mAb #2547
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | All | 14 kDa | Mouse | IgG |
Applications Key:
W=Western Blotting
Reactivity Key: All=All species expected
Specificity / Sensitivity
Tat (8C2) Mouse mAb detects recombinant Tat protein and Tat-tagged overexpressed protein. It does not cross-react with any other proteins.
Source / Purification
Monoclonal antibodies are produced by immunizing mice with a synthetic HIV-1 Tat-tag peptide (KLH-coupled).
Western Blotting
Western blot analysis of DRBD and Tat-tagged DRBD proteins (provided by Dr. Dowdy, UCSD) using Tat (8C2) Mouse mAb.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
A small peptide derived from the HIV-1 Tat protein has been used to internalize various types of molecules, from small particles to proteins, peptides and nucleic acids, into the cells. The 'transduction domain' or region conveying the cell penetrating properties of HIV Tat-derived peptide appears to be confined to a small (9 amino acid) stretch of basic amino acids, with the sequence RKKRRQRRR. The way this peptide enters cells is still under investigation (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
