Cell Signaling Technology

Product Pathways - Translational Control

EDC4/Ge-1 Antibody #2548

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H M Mk Endogenous 170 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

EDC4/Ge-1 Antibody detects endogenous levels of total EDC4/Ge-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human EDC4/Ge-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and Hep G2 cells using EDC4/Ge-1 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using EDC4/Ge-1 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

Background

Enhancer of mRNA decapping 4 (EDC4) was originally identified as the autoantigen Ge-1 from a Sjögren's syndrome patient later diagnosed with primary biliary cirrhosis (1). EDC4 (also known as HEDLS) was later identified as an essential component of cytoplasmic P-bodies responsible for mRNA decapping and degradation (2). Identified EDC4 protein is found as a pair of isoforms generated by alternative splicing and contains several WD domains and a putative nuclear localization signal. EDC4 co-localizes with other P-body decapping proteins such as DCP1A, DCP2 and GW182 (2,3). Experimental evidence suggests that EDC4 may be involved in miRNA-mediated translation repression (4).

  1. Bloch, D.B. et al. (1994) Clin Immunol Immunopathol 72, 380-9.
  2. Yu, J.H. et al. (2005) RNA 11, 1795-802.
  3. Fenger-Grøn, M. et al. (2005) Mol Cell 20, 905-15.
  4. Brodersen, P. et al. (2008) Science 320, 1185-90.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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