Product Pathways - Related Products
GFP Antibody #2555
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P | All | Transfected Only | 27 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: All=All species expected
Specificity / Sensitivity
GFP Antibody detects GFP-tagged proteins exogenously expressed in cells. Please note that the GFP tag adds approximately 27 kDa to the molecular weight of the fusion protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic GFP peptide (KLH-coupled). Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from COS-7 cells, mock transfected (lane 1) or expressing GFP-tagged fusion protein (lane 2), using GFP Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HCC827 cells control (left) or GFP-transfected (right) using GFP Antibody.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2372 β-Gal (14B7) Mouse mAb
- 2624 GST (26H1) Mouse mAb
- 2367 HA-Tag (6E2) Mouse mAb
- 2365 His-Tag Antibody
- 2396 MBP (8G1) Mouse mAb
- 2276 Myc-Tag (9B11) Mouse mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
