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GFP Antibody #2555

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P All Transfected Only 27 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: All=All species expected

Specificity / Sensitivity

GFP Antibody detects GFP-tagged proteins exogenously expressed in cells. Please note that the GFP tag adds approximately 27 kDa to the molecular weight of the fusion protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic GFP peptide (KLH-coupled). Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, mock transfected (lane 1) or expressing GFP-tagged fusion protein (lane 2), using GFP Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HCC827 cells control (left) or GFP-transfected (right) using GFP Antibody.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations (1).

  1. Miyawaki, A. et al. (2003) Curr. Opin. Chem. Biol. 7, 557-562.

Application References

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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