Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

LRP6 (C5C7) Rabbit mAb #2560

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H R Endogenous 180, 210 kDa Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  R=Rat
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

LRP6 (C5C7) Rabbit mAb detects endogenous levels of total LRP6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide. Blue Carrier-coupled) corresponding to residues surrounding Met1409 of human LRP6.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from HepG2, HeLa and Rat2 cells using LRP6 (C5C7) Rabbit mAb.

Background

LRP5 and LRP6 are single-pass transmembrane proteins belonging to the low-density-lipoprotein receptor (LDLR) related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain and proline-rich motifs in the cytoplasmic domain (1). They function as co-receptors for Wnt and are required for the canonical Wnt/β-catenin signaling pathway (2,3). LRP5 and LRP6 are highly homologous and have redundant roles during development (4,5). The activity of LRP5 and LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins (6,7). Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490 and Thr1493 by kinases such as GSK-3 and CK1 (8-10). The phosphorylated LRP6 recruits axin to the membrane, and presumably activates β-catenin signaling (8-10).

  1. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248, 879-888.
  2. Pinson, K.I. et al. (2000) Nature 407, 535-538.
  3. Tamai, K. et al. (2000) Nature 407, 530-535.
  4. Kelly, O.G. et al. (2004) Development 131, 2803-2815.
  5. He, X. et al. (2004) Development 131, 1663-1677.
  6. Semënov, M.V. et al. (2001) Curr Biol 11, 951-61.
  7. Bafico, A. et al. (2001) Nat. Cell Biol. 3, 683-668.
  8. Tamai, K. et al. (2004) Mol. Cell 13, 149-156.
  9. Zeng, X. et al. (2005) Nature 438, 873-877.
  10. Davidson, G. et al. (2005) Nature 438, 867-872.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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