Product Pathways - Cytoskeletal Signaling
RhoGDI Antibody #2564
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC F | H M R B | Endogenous | 26 kDa | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
RhoGDI Antibody detects endogenous levels of total RhoGDI protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to human RhoGDI. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of HeLa, NIH/3T3 and C6 cells, using RhoGDI Antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells, using RhoGDI Antibody (blue) compared to a nonspecific negative control antibody (red).
IC-IF
Immunofluorescent analysis of HeLa cells, showing cytosolic and perinuclear staining, using RhoGDI Antibody.
Background
Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI). RhoGDI affects Rho activity by inhibiting nucleotide exchange and membrane association, regulating activity and localization (Reviewed in 1, 2). The inhibitory and shuttling functions of RhoGDI have been uncoupled using mutant forms of RhoGDI (3). Phosphorylation of GDIs and/or GTPases can modulate their affinity for each other and, therefore, GTPase mediated signaling. PAK1 phosphorylation of RhoGDI at serines 101 and 174 causes release and activation of Rac1, but not RhoA (4).
- DerMardirossian, C. and Bokoch, G. (2005) Trends Cell Biol. 15, 356-363.
- Dovas, A. and Couchman, J.R. (2005) RhoGDI: multiple functions in the regulation of Rho family GTPase activities. Biochem. J. 390, 1-9.
- Dransart, E. et al. (2005) J. Biol. Chem. 280, 4674-4683.
- DerMardirossian, C. et al. (2004) Mol. Cell 15, 117-127.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
